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Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads
This study investigated the use of a newly developed chitosan-Ca pectinate microbead formulation for the colon-targeted delivery of anti-A/B toxin immunoglobulin of egg yolk (IgY) to inhibit toxin binding to colon mucosa cells. The effect of the three components (pectinate, calcium chloride, and chi...
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Published in: | AAPS PharmSciTech 2017-05, Vol.18 (4), p.1095-1103 |
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creator | Xing, Pingping Shi, Yanan Dong, Chuangchuang Liu, Hong Cheng, Yan Sun, Junbo Li, Dalei Li, Min Sun, Kaoxiang Feng, Dongxiao |
description | This study investigated the use of a newly developed chitosan-Ca pectinate microbead formulation for the colon-targeted delivery of anti-A/B toxin immunoglobulin of egg yolk (IgY) to inhibit toxin binding to colon mucosa cells. The effect of the three components (pectinate, calcium chloride, and chitosan) used for the microbead production was examined with the aim of identifying the optimal levels to improve drug encapsulation efficiency, swelling ratio, and cumulative IgY release rate. The optimized IgY-loaded bead component was pectin 5% (
w
/
v
), CaCl
2
3% (
w
/
v
), and chitosan 0.5% (
w
/
v
). Formulated beads were spherical with 1.2-mm diameter, and the drug loading was 45%. An
in vitro
release study revealed that chitosan-Ca pectinate microbeads inhibited IgY release in the upper gastrointestinal tract and significantly improved the site-specific release of IgY in the colon. An
in vivo
rat study demonstrated that 72.6% of biologically active IgY was released specifically in the colon. These results demonstrated that anti-A/B toxin IgY-loaded chitosan-Ca pectinate oral microbeads improved IgY release behavior
in vivo
, which could be used as an effective oral delivery platform for the biological treatment of
Clostridium difficile
infection (CDI). |
doi_str_mv | 10.1208/s12249-016-0656-2 |
format | article |
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w
/
v
), CaCl
2
3% (
w
/
v
), and chitosan 0.5% (
w
/
v
). Formulated beads were spherical with 1.2-mm diameter, and the drug loading was 45%. An
in vitro
release study revealed that chitosan-Ca pectinate microbeads inhibited IgY release in the upper gastrointestinal tract and significantly improved the site-specific release of IgY in the colon. An
in vivo
rat study demonstrated that 72.6% of biologically active IgY was released specifically in the colon. These results demonstrated that anti-A/B toxin IgY-loaded chitosan-Ca pectinate oral microbeads improved IgY release behavior
in vivo
, which could be used as an effective oral delivery platform for the biological treatment of
Clostridium difficile
infection (CDI).</description><identifier>ISSN: 1530-9932</identifier><identifier>EISSN: 1530-9932</identifier><identifier>DOI: 10.1208/s12249-016-0656-2</identifier><identifier>PMID: 27826799</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Antidiarrheals - administration & dosage ; Antidiarrheals - pharmacokinetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - metabolism ; Biochemistry ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Chitosan - chemistry ; Chitosan - pharmacology ; Clostridium Infections - drug therapy ; Colon - drug effects ; Colon - microbiology ; Drug Delivery Systems ; Enterotoxins - metabolism ; Immunoglobulins - administration & dosage ; Immunoglobulins - pharmacology ; Microspheres ; Pectins - administration & dosage ; Pectins - pharmacokinetics ; Pharmacology/Toxicology ; Pharmacy ; Rats ; Research Article ; Theme: Recent Trends in the Development of Chitosan-Based Drug Delivery Systems</subject><ispartof>AAPS PharmSciTech, 2017-05, Vol.18 (4), p.1095-1103</ispartof><rights>American Association of Pharmaceutical Scientists 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-3c25b1551bbfb2d926e9f117de71b10cc8ce3becd60d14b6e55129889d3ecfdb3</citedby><cites>FETCH-LOGICAL-c532t-3c25b1551bbfb2d926e9f117de71b10cc8ce3becd60d14b6e55129889d3ecfdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27826799$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xing, Pingping</creatorcontrib><creatorcontrib>Shi, Yanan</creatorcontrib><creatorcontrib>Dong, Chuangchuang</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Cheng, Yan</creatorcontrib><creatorcontrib>Sun, Junbo</creatorcontrib><creatorcontrib>Li, Dalei</creatorcontrib><creatorcontrib>Li, Min</creatorcontrib><creatorcontrib>Sun, Kaoxiang</creatorcontrib><creatorcontrib>Feng, Dongxiao</creatorcontrib><title>Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads</title><title>AAPS PharmSciTech</title><addtitle>AAPS PharmSciTech</addtitle><addtitle>AAPS PharmSciTech</addtitle><description>This study investigated the use of a newly developed chitosan-Ca pectinate microbead formulation for the colon-targeted delivery of anti-A/B toxin immunoglobulin of egg yolk (IgY) to inhibit toxin binding to colon mucosa cells. The effect of the three components (pectinate, calcium chloride, and chitosan) used for the microbead production was examined with the aim of identifying the optimal levels to improve drug encapsulation efficiency, swelling ratio, and cumulative IgY release rate. The optimized IgY-loaded bead component was pectin 5% (
w
/
v
), CaCl
2
3% (
w
/
v
), and chitosan 0.5% (
w
/
v
). Formulated beads were spherical with 1.2-mm diameter, and the drug loading was 45%. An
in vitro
release study revealed that chitosan-Ca pectinate microbeads inhibited IgY release in the upper gastrointestinal tract and significantly improved the site-specific release of IgY in the colon. An
in vivo
rat study demonstrated that 72.6% of biologically active IgY was released specifically in the colon. These results demonstrated that anti-A/B toxin IgY-loaded chitosan-Ca pectinate oral microbeads improved IgY release behavior
in vivo
, which could be used as an effective oral delivery platform for the biological treatment of
Clostridium difficile
infection (CDI).</description><subject>Animals</subject><subject>Antidiarrheals - administration & dosage</subject><subject>Antidiarrheals - pharmacokinetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - metabolism</subject><subject>Biochemistry</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>Clostridium Infections - drug therapy</subject><subject>Colon - drug effects</subject><subject>Colon - microbiology</subject><subject>Drug Delivery Systems</subject><subject>Enterotoxins - metabolism</subject><subject>Immunoglobulins - administration & dosage</subject><subject>Immunoglobulins - pharmacology</subject><subject>Microspheres</subject><subject>Pectins - administration & dosage</subject><subject>Pectins - pharmacokinetics</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Rats</subject><subject>Research Article</subject><subject>Theme: Recent Trends in the Development of Chitosan-Based Drug Delivery Systems</subject><issn>1530-9932</issn><issn>1530-9932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kDtvVDEQRq0IlCc_IA1ySWPwY-_D5eYSSKSgpNgUVJYfczeO7tob2xexFX8dRxtQqlQz0pz5NHMQOmf0M-O0_5IZ5wtJKGsJbZuW8AN0zBpBiZSCv3vVH6GTnB8p5YJJcYiOeNfztpPyGP0Z4hQDWem0hgIOf4XJ_4K0w3HE1-ufeLnWPuSChynmkrzz8wY7P47e-gnwKv72AS-xDg5fYLPDl8HqbZ4nXXwMuM6GB19i1oEMGt-BLT7oAviHtyka0C6fofejnjJ8eKmn6P7b5Wq4Ije336-H5Q2xjeCFCMsbw5qGGTMa7iRvQY6MdQ46Zhi1trcgDFjXUscWpoWKctn30gmwozPiFH3a525TfJohF7Xx2cI06QBxzor1QjLa9YtFRdkerTfmnGBU2-Q3Ou0Uo-rZu9p7V9W7evaueN35-BI_mw24_xv_RFeA74FcR2ENST3GOYX68hupfwHRoo8H</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Xing, Pingping</creator><creator>Shi, Yanan</creator><creator>Dong, Chuangchuang</creator><creator>Liu, Hong</creator><creator>Cheng, Yan</creator><creator>Sun, Junbo</creator><creator>Li, Dalei</creator><creator>Li, Min</creator><creator>Sun, Kaoxiang</creator><creator>Feng, Dongxiao</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads</title><author>Xing, Pingping ; Shi, Yanan ; Dong, Chuangchuang ; Liu, Hong ; Cheng, Yan ; Sun, Junbo ; Li, Dalei ; Li, Min ; Sun, Kaoxiang ; Feng, Dongxiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-3c25b1551bbfb2d926e9f117de71b10cc8ce3becd60d14b6e55129889d3ecfdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antidiarrheals - administration & dosage</topic><topic>Antidiarrheals - pharmacokinetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Toxins - metabolism</topic><topic>Biochemistry</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - pharmacology</topic><topic>Clostridium Infections - drug therapy</topic><topic>Colon - drug effects</topic><topic>Colon - microbiology</topic><topic>Drug Delivery Systems</topic><topic>Enterotoxins - metabolism</topic><topic>Immunoglobulins - administration & dosage</topic><topic>Immunoglobulins - pharmacology</topic><topic>Microspheres</topic><topic>Pectins - administration & dosage</topic><topic>Pectins - pharmacokinetics</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Rats</topic><topic>Research Article</topic><topic>Theme: Recent Trends in the Development of Chitosan-Based Drug Delivery Systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xing, Pingping</creatorcontrib><creatorcontrib>Shi, Yanan</creatorcontrib><creatorcontrib>Dong, Chuangchuang</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Cheng, Yan</creatorcontrib><creatorcontrib>Sun, Junbo</creatorcontrib><creatorcontrib>Li, Dalei</creatorcontrib><creatorcontrib>Li, Min</creatorcontrib><creatorcontrib>Sun, Kaoxiang</creatorcontrib><creatorcontrib>Feng, Dongxiao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>AAPS PharmSciTech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xing, Pingping</au><au>Shi, Yanan</au><au>Dong, Chuangchuang</au><au>Liu, Hong</au><au>Cheng, Yan</au><au>Sun, Junbo</au><au>Li, Dalei</au><au>Li, Min</au><au>Sun, Kaoxiang</au><au>Feng, Dongxiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads</atitle><jtitle>AAPS PharmSciTech</jtitle><stitle>AAPS PharmSciTech</stitle><addtitle>AAPS PharmSciTech</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>18</volume><issue>4</issue><spage>1095</spage><epage>1103</epage><pages>1095-1103</pages><issn>1530-9932</issn><eissn>1530-9932</eissn><abstract>This study investigated the use of a newly developed chitosan-Ca pectinate microbead formulation for the colon-targeted delivery of anti-A/B toxin immunoglobulin of egg yolk (IgY) to inhibit toxin binding to colon mucosa cells. The effect of the three components (pectinate, calcium chloride, and chitosan) used for the microbead production was examined with the aim of identifying the optimal levels to improve drug encapsulation efficiency, swelling ratio, and cumulative IgY release rate. The optimized IgY-loaded bead component was pectin 5% (
w
/
v
), CaCl
2
3% (
w
/
v
), and chitosan 0.5% (
w
/
v
). Formulated beads were spherical with 1.2-mm diameter, and the drug loading was 45%. An
in vitro
release study revealed that chitosan-Ca pectinate microbeads inhibited IgY release in the upper gastrointestinal tract and significantly improved the site-specific release of IgY in the colon. An
in vivo
rat study demonstrated that 72.6% of biologically active IgY was released specifically in the colon. These results demonstrated that anti-A/B toxin IgY-loaded chitosan-Ca pectinate oral microbeads improved IgY release behavior
in vivo
, which could be used as an effective oral delivery platform for the biological treatment of
Clostridium difficile
infection (CDI).</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27826799</pmid><doi>10.1208/s12249-016-0656-2</doi><tpages>9</tpages></addata></record> |
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subjects | Animals Antidiarrheals - administration & dosage Antidiarrheals - pharmacokinetics Bacterial Proteins - metabolism Bacterial Toxins - metabolism Biochemistry Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biomedical and Life Sciences Biomedicine Biotechnology Chitosan - chemistry Chitosan - pharmacology Clostridium Infections - drug therapy Colon - drug effects Colon - microbiology Drug Delivery Systems Enterotoxins - metabolism Immunoglobulins - administration & dosage Immunoglobulins - pharmacology Microspheres Pectins - administration & dosage Pectins - pharmacokinetics Pharmacology/Toxicology Pharmacy Rats Research Article Theme: Recent Trends in the Development of Chitosan-Based Drug Delivery Systems |
title | Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads |
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