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Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads

This study investigated the use of a newly developed chitosan-Ca pectinate microbead formulation for the colon-targeted delivery of anti-A/B toxin immunoglobulin of egg yolk (IgY) to inhibit toxin binding to colon mucosa cells. The effect of the three components (pectinate, calcium chloride, and chi...

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Published in:AAPS PharmSciTech 2017-05, Vol.18 (4), p.1095-1103
Main Authors: Xing, Pingping, Shi, Yanan, Dong, Chuangchuang, Liu, Hong, Cheng, Yan, Sun, Junbo, Li, Dalei, Li, Min, Sun, Kaoxiang, Feng, Dongxiao
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creator Xing, Pingping
Shi, Yanan
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Li, Min
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description This study investigated the use of a newly developed chitosan-Ca pectinate microbead formulation for the colon-targeted delivery of anti-A/B toxin immunoglobulin of egg yolk (IgY) to inhibit toxin binding to colon mucosa cells. The effect of the three components (pectinate, calcium chloride, and chitosan) used for the microbead production was examined with the aim of identifying the optimal levels to improve drug encapsulation efficiency, swelling ratio, and cumulative IgY release rate. The optimized IgY-loaded bead component was pectin 5% ( w / v ), CaCl 2 3% ( w / v ), and chitosan 0.5% ( w / v ). Formulated beads were spherical with 1.2-mm diameter, and the drug loading was 45%. An in vitro release study revealed that chitosan-Ca pectinate microbeads inhibited IgY release in the upper gastrointestinal tract and significantly improved the site-specific release of IgY in the colon. An in vivo rat study demonstrated that 72.6% of biologically active IgY was released specifically in the colon. These results demonstrated that anti-A/B toxin IgY-loaded chitosan-Ca pectinate oral microbeads improved IgY release behavior in vivo , which could be used as an effective oral delivery platform for the biological treatment of Clostridium difficile infection (CDI).
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subjects Animals
Antidiarrheals - administration & dosage
Antidiarrheals - pharmacokinetics
Bacterial Proteins - metabolism
Bacterial Toxins - metabolism
Biochemistry
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biomedical and Life Sciences
Biomedicine
Biotechnology
Chitosan - chemistry
Chitosan - pharmacology
Clostridium Infections - drug therapy
Colon - drug effects
Colon - microbiology
Drug Delivery Systems
Enterotoxins - metabolism
Immunoglobulins - administration & dosage
Immunoglobulins - pharmacology
Microspheres
Pectins - administration & dosage
Pectins - pharmacokinetics
Pharmacology/Toxicology
Pharmacy
Rats
Research Article
Theme: Recent Trends in the Development of Chitosan-Based Drug Delivery Systems
title Colon-Targeted Delivery of IgY Against Clostridium difficile Toxin A and B by Encapsulation in Chitosan-Ca Pectinate Microbeads
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