Hepatic BSCL2 (Seipin) Deficiency Disrupts Lipid Droplet Homeostasis and Increases Lipid Metabolism via SCD1 Activity

• Published in: Lipids 2017-02, Vol.52 (2), p.129-150
• Main Authors: Lounis, Mohamed Amine, Lalonde, Simon, Rial, Sabri Ahmed, Bergeron, Karl-F., Ralston, Jessica C., Mutch, David M., Mounier, Catherine
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; BSCL2; Fatty acid uptake; Gene Knock-In Techniques; Gene Knockdown Techniques; GTP-Binding Protein gamma Subunits - deficiency; Hep G2 Cells; Hepatocytes - cytology; Hepatocytes - metabolism; Homeostasis; Humans; Insulin - metabolism; Insulin sensitivity; Life Sciences; Lipid droplets; Lipid Droplets - metabolism; Lipid Metabolism; Lipidology; Lipogenesis; Medical Biochemistry; Medicinal Chemistry; Metabolic disorders; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Organelle Size; Original Article; Phosphorylation; Rats; SCD1; Seipin; Stearoyl-CoA Desaturase - genetics; Stearoyl-CoA Desaturase - metabolism
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-016-4210-5

Berardinelli-Seip congenital lipodystrophy (BSCL) is an autosomal recessive disorder. The more severe form, designated BSCL2, arises due to mutations in the BSCL2 gene. Patients with BSCL2, as well as Bscl2 −/− mice, have a near total absence of body fat, an organomegaly, and develop metabolic disorders including insulin resistance and hepatic steatosis. The function of the Seipin (BSCL2) protein remains poorly understood. Several lines of evidence have indicated that Seipin may have distinct functions in adipose versus non-adipose cells. Here we present evidence that BSCL2 / Bscl2 plays a role in lipid droplet (LD) biogenesis and homeostasis in primary and cultured hepatocytes. Our results show that decreasing BSCL2 / Bscl2 expression in hepatocytes increases the number and size of LD, as well as the expression of genes implicated in their formation and stability. We also show that knocking down SCD1 expression reverses the phenotype associated with Seipin deficiency. Interestingly, BSCL2 knockdown induces SCD1 expression and activity, potentially leading to increased basal phosphorylation of proteins involved in the insulin signaling cascade, as well as further increasing fatty acid uptake and de novo lipogenesis. In conclusion, our results suggest that a hepatic BSCL2 / Bscl2 deficiency induces the increase and expansion of LD, potentially via increased SCD1 activity.