Isolation and molecular characterization of Clostridium perfringens from healthy Merino lambs in Patagonia region, Argentina

The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in day-old lambs. Even though the majority of the isolates were characterized as bein...

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Bibliographic Details
Published in:Anaerobe 2017-02, Vol.43, p.35-38
Main Authors: Mignaqui, A.C., Marcellino, R.B., Ronco, T., Pappalardo, J.S., Nonnemann, B., Pedersen, K., Robles, C.A.
Format: Article
Language:English
Subjects:
Animals
Argentina - epidemiology
Clostridium Infections - epidemiology
Clostridium Infections - microbiology
Clostridium Infections - veterinary
Clostridium perfringens
Clostridium perfringens - genetics
Clostridium perfringens - isolation & purification
Electrophoresis, Gel, Pulsed-Field - veterinary
Enterotoxaemia
Female
Gastrointestinal Diseases - epidemiology
Gastrointestinal Diseases - microbiology
Gastrointestinal Diseases - veterinary
Genetic Variation
Lambs
PCR
PFGE
Polymerase Chain Reaction - veterinary
Prevalence
Sheep
Sheep Diseases - epidemiology
Sheep Diseases - microbiology
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Summary:The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in day-old lambs. Even though the majority of the isolates were characterized as being of type A, types C and D were also isolated. Furthermore, a high genetic diversity was observed by PFGE among the type A isolates.
ISSN:1075-9964
1095-8274
DOI:10.1016/j.anaerobe.2016.10.015