Hormonal profiling: Development of a simple method to extract and quantify phytohormones in complex matrices by UHPLC–MS/MS

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-01, Vol.1040, p.239-249
• Main Authors: Delatorre, Carolina, Rodríguez, Ana, Rodríguez, Lucía, Majada, Juan P., Ordás, Ricardo J., Feito, Isabel
• Format: Article
• Language: English
• Subjects: Chromatography, High Pressure Liquid - economics; Chromatography, High Pressure Liquid - methods; Complex matrix; Liquid-Liquid Extraction - methods; Pinus - chemistry; Pinus pinaster; Plant Extracts - chemistry; Plant growth regulator; Plant Growth Regulators - analysis; Plant Growth Regulators - isolation & purification; Solvents - chemistry; Tandem mass spectrometry; Tandem Mass Spectrometry - economics; Tandem Mass Spectrometry - methods; UHPLC
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2016.11.007

•Development of a fast and simple method for plant growth regulators analysis.•Avoids the use of tedious and highly time consuming steps.•Feasible to obtain 20 different compounds in only one round of extraction.•Capable of quantify in one injection all analytes extracted.•Optimized in complex plant matrices. Plant growth regulators (PGRs) are very different chemical compounds that play essential roles in plant development and the regulation of physiological processes. They exert their functions by a mechanism called cross-talk (involving either synergistic or antagonistic actions) thus; it is for great interest to study as many PGRs as possible to obtain accurate information about plant status. Much effort has been applied to develop methods capable of analyze large numbers of these compounds but frequently excluding some chemical families or important PGRs within each family. In addition, most of the methods are specially designed for matrices easy to work with. Therefore, we wanted to develop a method which achieved the requirements lacking in the literature and also being fast and reliable. Here we present a simple, fast and robust method for the extraction and quantification of 20 different PGRs using UHPLC–MS/MS optimized in complex matrices.