Identification and Characterization of the Human Protein Kinase-like Gene NTKL: Mitosis-Specific Centrosomal Localization of an Alternatively Spliced Isoform

Although the centrosome has an essential role in mitosis, its molecular components have not been fully elucidated. Here, we describe the molecular cloning and characterization of the human gene NTKL, which encodes an evolutionarily conserved kinase-like protein. NTKL mRNA is found ubiquitously in hu...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2002-06, Vol.79 (6), p.760-767
Main Authors: Kato, Masahiro, Yano, Ken-ichi, Morotomi-Yano, Keiko, Saito, Hiroko, Miki, Yoshio
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Cell cycle, cell proliferation
Cell physiology
Centrosome - metabolism
Fundamental and applied biological sciences. Psychology
Genes. Genome
Humans
Mitosis - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Organ Specificity
Phylogeny
Protein Isoforms - genetics
Protein Isoforms - metabolism
Protein Kinases - genetics
Protein Kinases - isolation & purification
Protein Kinases - metabolism
Sequence Analysis, DNA
Transcription Factors
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Summary:Although the centrosome has an essential role in mitosis, its molecular components have not been fully elucidated. Here, we describe the molecular cloning and characterization of the human gene NTKL, which encodes an evolutionarily conserved kinase-like protein. NTKL mRNA is found ubiquitously in human tissues. NTKL is located on 11q13 and is mapped around chromosomal breakpoints found in several carcinomas, suggesting that NTKL dysfunction may be involved in carcinogenesis. Alternative splicing generates two variant forms of NTKL mRNA that encode protein isoforms with internal deletions. When fused to green fluorescent protein, the full-length product and one of the variant proteins are found in cytoplasm. The other variant product also exists in the cytoplasm during interphase, but is found in the centrosomes during mitosis. Endogenous NTKL protein is also localized to the centrosomes during mitosis. This cell-cycle-dependent centrosomal localization suggests that NTKL is involved in centrosome-related cellular functions.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.2002.6774