All trans retinoic acid depresses the content and activity of the mitochondrial ATP synthase in human keratinocytes

Proteomic analysis shows that treatment of keratinocytes cultures with all trans retinoic acid (ATRA), under condition in which it inhibits cell growth, results in marked decrease of the level of the F1-β subunit of the catalytic sector of the mitochondrial FoF1 ATP synthase complex. Enzymatic analy...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2017-01, Vol.482 (2), p.301-304
Main Authors: Papa, F., Lippolis, R., Sardaro, N., Gnoni, A., Scacco, S.
Format: Article
Language:English
Subjects:
All-trans retinoic acid
ATP synthase
Cell Proliferation - drug effects
Cell Proliferation - physiology
Cells, Cultured
Dose-Response Relationship, Drug
Down-Regulation - drug effects
Down-Regulation - physiology
Enzyme Activation - drug effects
Human keratinocytes
Humans
Keratinocytes - cytology
Keratinocytes - enzymology
Mass spectrometry
Mitochondrial Proton-Translocating ATPases - biosynthesis
Proteomics
Tretinoin - administration & dosage
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Summary:Proteomic analysis shows that treatment of keratinocytes cultures with all trans retinoic acid (ATRA), under condition in which it inhibits cell growth, results in marked decrease of the level of the F1-β subunit of the catalytic sector of the mitochondrial FoF1 ATP synthase complex. Enzymatic analysis shows in ATRA-treated keratinocytes a consistent depression of the ATPase activity, with decreased olygomycin sensitivity, indicating an overall alteration of the ATP synthase complex. These findings, together with the previously reported inhibition of respiratory complex I, show that depression of the activity of oxidative phosphorylation enzymes is involved in the cell growth inhibitory action of ATRA.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2016.11.058