DPPH·–luminol chemiluminescence system and its application in the determination of scutellarin in pharmaceutical injections and rat plasma with flow injection analysis

• Published in: Luminescence (Chichester, England) England), 2017-06, Vol.32 (4), p.588-595
• Main Authors: Yao, Hong, Huang, Xiaomei, Shi, Peiying, Lin, Zhen, Zhu, Meilan, Liu, Ailin, Lin, Xinhua, Tang, Yuhai
• Format: Article
• Language: English
• Subjects: Analytical methods; Animals; Apigenin - analysis; Apigenin - blood; Biphenyl Compounds - chemistry; Chemiluminescence; Detection; DPPH; flow injection; Flow injection analysis; Flow Injection Analysis - methods; Glucuronates - analysis; Glucuronates - blood; Injection; Limit of Detection; Luminescent Agents - chemistry; Luminescent Measurements - methods; Luminol - chemistry; pharmaceutical injections; Pharmaceuticals; Picrates - chemistry; Rapid flow; rat plasma; Rats, Sprague-Dawley; Reproducibility of Results; scutellarin; Sequencing; Sodium hydroxide; Stability
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.3225

In this article, a DPPH·–luminol chemiluminescence (CL) system was reported and the CL mechanism was discussed according to the CL kinetic properties after sequence injecting DPPH· into the DPPH·–luminol reaction mixture. It was observed that scutellarin could inhibit the CL response of the DPPH·–luminol system. Based on this observation, a simple and rapid flow injection CL method was developed for the determination of scutellarin using the inhibition effect in alkaline medium. The optimized chemical conditions for the CL reaction were 5 × 10−6 mol/L DPPH· and 1.0 × 10−4 mol/L luminol in 0.01 mol/L NaOH. Under optimized conditions, the CL intensity was inversely proportional to the concentration of scutellarin over the ranges 5–2000 and 40–3200 ng/ml in pharmaceutical injection and rat plasma, respectively. The limits of detection (S/N = 3) were 5 and 40 ng/ml in preparations and rat plasma, respectively. Furthermore, the precision, recovery and stability of the validated method were acceptable for the determination of scutellarin in both pharmaceutical injections and rat plasma. The presented method was successfully applied in the determination of scutellarin in pharmaceutical injections and real rat plasma samples.