Role of G Protein beta 3 Subunit C825T and HLA Class II Polymorphisms in the Immune Response after HBV Vaccination

• Published in: Virology (New York, N.Y.) N.Y.), 2002-06, Vol.297 (2), p.245-252
• Main Authors: Lindemann, M, Barsegian, V, Siffert, W, Ferencik, S, Roggendorf, M, Grosse-Wilde, H
• Format: Article
• Language: English
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1006/viro.2002.1467

The G protein beta 3 (GNB3) subunit and HLA are candidate genes predictive of immune response capacity. We therefore studied the influence of both gene systems on cellular and humoral immunity against hepatitis B virus (HBV) in 79 HBV booster-vaccinated healthy volunteers and an independent group of 77 probands after HBV basic immunization. Following booster vaccination, lymphocyte in vitro proliferation after stimulation with HBV surface antigen was 2.5-fold increased in GNB3 825T (TC + TT) vs CC allele carriers (P = 0.01) and was not influenced by HLA-DRB1 or DQB1 alleles. In addition, anti-HBs antibody titers in both groups were 2-fold increased in TC vs CC and decreased in TT vs CC allele carriers. However, antibody titers after HBV booster immunization were elevated in HLA-DQB1*0301 carriers (P corrected = 0.027). In summary, the GNB3 825T allele appears as a marker particularly predictive of cellular and HLA-DQB1*0301 of humoral immune responses following HBV vaccination. [copy ] 2002 Elsevier Science (USA).