Some proteins of M. tuberculosis that localise to the nucleus of THP-1-derived macrophages

• Published in: Tuberculosis (Edinburgh, Scotland) Scotland), 2016-12, Vol.101, p.75-78
• Main Authors: Agrawal, Atul Kumar, Ranjan, Rajeev, Chandra, Sharat, Rout, Tofan Kumar, Misra, Amit, Reddy, Thota Jagadeshwar
• Format: Article
• Language: English
• Subjects: Bacterial proteins; Bacterial Proteins - metabolism; Binding sites; Cell Nucleus - metabolism; Deoxyribonucleic acid; DNA; DNA-binding proteins; DNA-Binding Proteins - metabolism; Gene expression; Host-Pathogen Interactions - physiology; Humans; Infectious Disease; Lysates; Macrophages; Macrophages - metabolism; Macrophages - microbiology; Mass spectrometry; Mass spectroscopy; Moonlighting; Mycobacterium tuberculosis - metabolism; Mycobacterium tuberculosis - physiology; Nano-LC/MS; Nuclear proteome; Nuclei; Protein interaction; Proteins; Protein–protein interactions; Proteomics; Pulmonary/Respiratory; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Time dependence; Tuberculosis; Tuberculosis - metabolism; Western blotting
• ISSN: 1472-9792; 1873-281X
• DOI: 10.1016/j.tube.2016.07.013

Summary Host-pathogen dialectics in tuberculosis (TB) via DNA-protein interactions are emerging. We investigated whether proteins produced by Mycobacterium tuberculosis (Mtb) could translocate to the host nucleus. Using lysates of nuclei purified from Mtb-infected THP-1-derived macrophages, we identified at least 15 proteins of Mtb-origin by electrophoretic and chromatographic separation and mass spectrometry. Western blotting confirmed time-dependent accumulation of Mtb EF-Tu, GroEL, GroES and MtrA in the host nucleus. MtrA could pull down at least 16 host proteins. Mtb proteins may have moonlighting functions that affect host gene expression.