Mycobacterium smegmatis proteoliposome induce protection in a murine progressive pulmonary tuberculosis model

• Published in: Tuberculosis (Edinburgh, Scotland) Scotland), 2016-12, Vol.101, p.44-48
• Main Authors: Tirado, Yanely, Puig, Alina, Alvarez, Nadine, Borrero, Reinier, Aguilar, Alicia, Camacho, Frank, Reyes, Fatima, Fernandez, Sonsire, Perez, Jose Luis, Acevedo, Reynaldo, Espinoza, Dulce Mata, Barrios Payan, Jorge Alberto, Garcia, Maria de los A, Kadir, Ramlah, Sarmiento, María E, Hernandez-Pando, Rogelio, Norazmi, Mohd-Nor, Acosta, Armando
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic; Alum; Alum Compounds; Aluminum sulfate; Animal models; Animals; Antigens; Bacillus Calmette-Guerin vaccine; Bacterial infections; Bacterial Load; BCG; BCG Vaccine; Challenge; Children; Disease Models, Animal; Disease Progression; Disease transmission; Immunization; Infectious Disease; Lungs; Male; Mice; Mice, Inbred BALB C; Morbidity; Mycobacterium smegmatis; Mycobacterium smegmatis - immunology; Mycobacterium tuberculosis - growth & development; Mycobacterium tuberculosis - isolation & purification; Pneumonia, Bacterial - microbiology; Pneumonia, Bacterial - pathology; Pneumonia, Bacterial - prevention & control; Proteolipids - immunology; Proteoliposomes; Pulmonary/Respiratory; Respiratory therapy; Rodents; Tuberculosis; Tuberculosis Vaccines; Tuberculosis, Pulmonary - immunology; Tuberculosis, Pulmonary - microbiology; Tuberculosis, Pulmonary - pathology; Tuberculosis, Pulmonary - prevention & control; Vaccines
• ISSN: 1472-9792; 1873-281X
• DOI: 10.1016/j.tube.2016.07.017

Summary Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb.