Microbiological assessment of aerosol generated during debond of fixed orthodontic appliances

Introduction The aims of this study were to describe bacterial load and diversity of the aerosol created during enamel cleanup after the removal of fixed orthodontic appliances and to assess the effect of a preprocedural mouth rinse. Methods The study involved the sampling of ambient air adjacent to...

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Bibliographic Details
Published in:American journal of orthodontics and dentofacial orthopedics 2016-11, Vol.150 (5), p.831-838
Main Authors: Dawson, Michael, Soro, Valeria, Dymock, David, Price, Robert, Griffiths, Helen, Dudding, Tom, Sandy, Jonathan R, Ireland, Anthony J
Format: Article
Language:English
Subjects:
Aerosols
Bacteria - isolation & purification
Chlorhexidine - therapeutic use
Dental Debonding - adverse effects
Dental Debonding - instrumentation
Dental Debonding - methods
Dental Enamel - microbiology
Dentistry
Electrophoresis - methods
Humans
Mouthwashes - therapeutic use
Orthodontic Appliances - microbiology
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Summary:Introduction The aims of this study were to describe bacterial load and diversity of the aerosol created during enamel cleanup after the removal of fixed orthodontic appliances and to assess the effect of a preprocedural mouth rinse. Methods The study involved the sampling of ambient air adjacent to the patient's mouth during adhesive removal using a slow-speed handpiece and a spiral fluted tungsten carbide bur without water irrigation. Sampling was carried out during enamel cleanup with or without a preprocedural mouth rinse of either sterile water or chlorhexidine. Airborne particles were collected using a viable inertial impactor simulating the human respiratory tree. The bacteria collected were analyzed using both culture and molecular techniques. Results Bacteria produced during debond and enamel cleanup can reach all levels of the respiratory tree. The use of a preprocedural mouth rinse, either sterile water or chlorhexidine, increased the numbers and diversity of the bacteria in the air. Conclusions When using a slow-speed handpiece and a spiral fluted tungsten carbide bur for enamel cleanup after orthodontic treatment, the bacterial load and diversity of the aerosol produced are lower when a preprocedural mouth rinse is not used.
ISSN:0889-5406
1097-6752
DOI:10.1016/j.ajodo.2016.04.022