Uptake of silica nanoparticles in the brain and effects on neuronal differentiation using different in vitro models

Abstract Nanomedicine offers a promising tool for therapies of brain diseases, but they may be associated with potential adverse effects. The aim of this study was to investigate the uptake of silica-nanoparticles engineered for laser-tissue soldering in the brain using SH-SY5Y cells, dissociated an...

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Bibliographic Details
Published in:Nanomedicine 2017-04, Vol.13 (3), p.1195-1204
Main Authors: Ducray, A.D, Stojiljkovic, A, Möller, A, Stoffel, M.H, Widmer, H.R, Frenz, M, Mevissen, M
Format: Article
Language:English
Subjects:
Animals
Brain - cytology
Brain - drug effects
Brain - metabolism
Cell Line
Cell Survival - drug effects
Cells, Cultured
Hippocampus - cytology
Hippocampus - drug effects
Hippocampus - metabolism
Humans
Internal Medicine
Microglia - drug effects
Microglia - metabolism
Nanomedicine
Nanoparticle uptake
Nanoparticles - analysis
Nanoparticles - metabolism
Nanoparticles - toxicity
Neurodifferentiation
Neurogenesis - drug effects
Neuroinflammation
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
Rats
Rats, Wistar
Silicon Dioxide - metabolism
Silicon Dioxide - pharmacokinetics
Silicon Dioxide - toxicity
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Summary:Abstract Nanomedicine offers a promising tool for therapies of brain diseases, but they may be associated with potential adverse effects. The aim of this study was to investigate the uptake of silica-nanoparticles engineered for laser-tissue soldering in the brain using SH-SY5Y cells, dissociated and organotypic slice cultures from rat hippocampus. Nanoparticles were predominantly taken up by microglial cells in the hippocampal cultures but nanoparticles were also found in differentiated SH-SY5Y cells. The uptake was time- and concentration-dependent in primary hippocampal cells. Transmission electron microscopy experiments demonstrated nanoparticle aggregates and single particles in the cytoplasm. Nanoparticles were found in the endoplasmic reticulum, but not in other cellular compartments. Nanoparticle exposure did not impair cell viability and neuroinflammation in primary hippocampal cultures at all times investigated. Neurite outgrowth was not significantly altered in SH-SY5Y cells, but the neuronal differentiation markers indicated a reduction in neuronal differentiation induction after nanoparticle exposure.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2016.11.001