Somatic hybridization by electrofusion of banana protoplasts

Somatic hybridization between triploid and diploid bananas was attempted by using protoplast electrofusion and nurse culture techniques. Protoplasts from embryogenic cell suspensions of 'Maçã' (Musa sp. AAB group) were fused with protoplasts from nonembryogenic calli of`Lidi' (Musa sp...

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Bibliographic Details
Published in:Euphytica 2002-01, Vol.125 (3), p.317-324
Main Authors: MATSUMOTO, Kazumitsu, VILARINHOS, Alberto Duarte, OKA, Seibi
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Bananas
Biological and medical sciences
Cell culture
Cell suspensions
Culture techniques
Diploids
Electrofusion
Embryogenesis
Embryonic growth stage
Flow cytometry
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
Genomics
Haploidy, in vitro culture applications, somatic hybrids
Hybridization
Hybrids
Nurse cells
Plant breeding: fundamental aspects and methodology
Plantlets
Ploidy
Polymerase chain reaction
Polymorphism
Protoplasts
Random amplified polymorphic DNA
Rice
Somatic embryogenesis
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Summary:Somatic hybridization between triploid and diploid bananas was attempted by using protoplast electrofusion and nurse culture techniques. Protoplasts from embryogenic cell suspensions of 'Maçã' (Musa sp. AAB group) were fused with protoplasts from nonembryogenic calli of`Lidi' (Musa sp. AA group). Direct somatic embryogenesis was observed when the fusion-treated protoplasts were cultured with rice nurse cells (Oryza sativa L. A-58 line). Somatic hybrids were identified by using random amplified polymorphic DNA (RAPD) markers. Forty-four primers were tested for polymorphism between the two parental varieties, and eleven of them showed polymorphism. Among the 13 regenerated plants from one experiment, 11 (85%) were identified as somatic hybrids by the presence of both parent bands, and 2 showed only the 'Lidi'-band patterns. From the other experiment, 5 of 11 regenerated plantlets (45%) were identified as somatic hybrids. Flow cytometric ploidy analyses showed different ploidy levels of the somatic hybrids.
ISSN:0014-2336
1573-5060
DOI:10.1023/A:1016071624090