Cypermethrin-induced plasma membrane perturbation on erythrocytes from rats: reduction of fluidity in the hydrophobic core and in glutathione peroxidase activity

The effects of treatment with the synthetic insecticide cypermethrin on plasma membrane fluidity, lipid peroxidation and antioxidant status in rat erythrocytes were investigated. Rats were treated by gavage with a low dose (12.5 mg/kg body weight per day) of cypermethrin in corn oil for 60 days. DPH...

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Bibliographic Details
Published in:Toxicology (Amsterdam) 2002-06, Vol.175 (1), p.91-101
Main Authors: Gabbianelli, Rosita, Falcioni, Giancarlo, Nasuti, Cinzia, Cantalamessa, Franco
Format: Article
Language:English
Subjects:
Animals
Antioxidant enzymes
Biological and medical sciences
Catalase - blood
Cell Membrane - drug effects
Cell Membrane - enzymology
Cell Membrane - physiology
Cypermethrin
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - enzymology
Erythrocyte Membrane - physiology
Fluorescence Polarization
Glutathione Peroxidase - blood
Insecticides - toxicity
Lipid oxidation
Lipid Peroxides - biosynthesis
Male
Medical sciences
Membrane fluidity
Membrane Fluidity - drug effects
Membrane Proteins - metabolism
Methemoglobin - metabolism
Pesticides, fertilizers and other agrochemicals toxicology
Pyrethrins - toxicity
Rat erythrocytes
Rats
Rats, Wistar
Superoxide Dismutase - blood
Toxicology
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Summary:The effects of treatment with the synthetic insecticide cypermethrin on plasma membrane fluidity, lipid peroxidation and antioxidant status in rat erythrocytes were investigated. Rats were treated by gavage with a low dose (12.5 mg/kg body weight per day) of cypermethrin in corn oil for 60 days. DPH and TMA-DPH fluorescence anisotropy experiments show that cypermethrin treatment, compared with controls, induced a significant decrease in erythrocyte membrane fluidity measured by DPH, while no changes were observed using TMA-DPH. Cypermethrin treatment also induced a significant increase in the lipid peroxidation, measured by the formation of conjugated dienes. The increased oxidative stress resulted in a significant decrease in the acitivity of glutathione peroxidase. The results are discussed in terms of preferential localization of cypermethrin in the hydrophobic core of the membrane, where it increases lipid packing and consequently decreases membrane fluidity.
ISSN:0300-483X
1879-3185
DOI:10.1016/S0300-483X(02)00058-6