Role of Tissue-Specific Stem and Progenitor Cells in the Regeneration of the Pancreas and Testicular Tissue in Diabetic Disorders

• Published in: Bulletin of experimental biology and medicine 2016-11, Vol.162 (1), p.146-152
• Main Authors: Skurikhin, E. G., Pakhomova, A. V., Ermakova, N. N., Pershina, O. V., Krupin, V. A., Pan, E. S., Kudryashova, A. I., Ermolaeva, L. A., Dygai, A. M.
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antigens, CD - genetics; Antigens, CD - immunology; Beta cells; Biomedical and Life Sciences; Biomedicine; Blood Glucose - immunology; Blood Glucose - metabolism; CD29 antigen; CD45 antigen; CD90 antigen; Cell Biology; Diabetes mellitus; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - immunology; Diabetes Mellitus, Experimental - pathology; Diet, High-Fat; Epithelial cells; Female; Gene Expression; Glucose metabolism; High fat diet; Hypogonadism; Hypogonadism - chemically induced; Hypogonadism - genetics; Hypogonadism - immunology; Hypogonadism - pathology; Immunophenotyping; Infertility; Insulin; Insulin resistance; Insulin-Secreting Cells - immunology; Insulin-Secreting Cells - pathology; Interferon; Interferon-gamma - genetics; Interferon-gamma - immunology; Interleukin 17; Interleukin 2; Interleukin 23; Interleukin-17 - genetics; Interleukin-17 - immunology; Interleukin-2 - genetics; Interleukin-2 - immunology; Interleukin-23 - genetics; Interleukin-23 - immunology; Internal Medicine; Laboratory Medicine; Male; Mesenchymal stem cells; Mesenchyme; Mice; Mice, Inbred C57BL; Organ Specificity; Pancreas; Pancreas - immunology; Pancreas - pathology; Pathology; Physiological aspects; Regeneration - genetics; Regeneration - immunology; Reproductive system; Rodents; Spermatogenesis; Spermatogenesis - genetics; Spermatogonia - immunology; Spermatogonia - pathology; Stem cell transplantation; Stem cells; Stem Cells - immunology; Stem Cells - pathology; Streptozocin; Stromal cells; Testes; Testis - immunology; Testis - pathology; Testosterone; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Type 2 diabetes
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-016-3564-2

Using the model of hypogonadism in C57Bl/6 male mice, we showed that injection of streptozotocin to newborn animals and high-fat diet induced serum IFN-γ and IL-17 elevation, glucose metabolism disturbances, insulin resistance, destructive changes of the Langerhans islets (deficit of PDX1 + β cells), while the number of oligopotent β cell precursors (CD45 — TER119 — CD133 + CD49f low ) increased. Diabetes played the role of an inducer of testicular tissue inflammation (pan-hemopoietic cell infiltration, increase of IL-2, IL-17, and IL-23 content) and reproductive system disturbances in mice (decrease in free testosterone concentration, suppression of spermatogenesis, and infertility). The development of hypogonadism was paralleled by an increase in the count of spermatogonial stem cells (CD117 + CD29 + CD90 + ), multipotent mesenchymal stromal cells (CD45 — CD31 — CD90 + CD106 + ), hemangiogenesis precursors (CD45 — CD117 + Flk1 + ), and epithelial cells (CD45 — CD31 — CD49f + CD326 + ).