Negative pressure wound therapy inhibits inflammation and upregulates activating transcription factor‐3 and downregulates nuclear factor‐κB in diabetic patients with foot ulcerations

Background Negative pressure wound therapy (NPWT) is one of the most important treatments for diabetic foot, but the underlying mechanisms of its benefits still remain elusive. This study aims to evaluate the inflammatory signals involved in the effects of negative pressure therapy on diabetic foot...

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Bibliographic Details
Published in:Diabetes/metabolism research and reviews 2017-05, Vol.33 (4), p.n/a
Main Authors: Wang, T., He, R., Zhao, J., Mei, J.C., Shao, M.Z., Pan, Y., Zhang, J., Wu, H.S., Yu, M., Yan, W.C., Liu, L.M., Liu, F., Jia, W.P.
Format: Article
Language:English
Subjects:
Activating Transcription Factor 3 - metabolism
Aged
ATF‐3
diabetic foot
Diabetic Foot - metabolism
Diabetic Foot - therapy
Down-Regulation
Female
Humans
inflammation
Inflammation - metabolism
Inflammation - pathology
Inflammation - therapy
Interleukin-6 - metabolism
Male
Middle Aged
Negative-Pressure Wound Therapy
NF-kappa B - metabolism
Nitric Oxide Synthase Type II - metabolism
nuclear factor‐κB
Treatment Outcome
Tumor Necrosis Factor-alpha - metabolism
Up-Regulation
Wound Healing - physiology
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Summary:Background Negative pressure wound therapy (NPWT) is one of the most important treatments for diabetic foot, but the underlying mechanisms of its benefits still remain elusive. This study aims to evaluate the inflammatory signals involved in the effects of negative pressure therapy on diabetic foot ulcers. Methods We enrolled 22 patients with diabetic foot ulceration, 11 treated with NPWT and the other 11 treated with traditional debridement. All patients were treated and observed for 1 week. Granulation tissues were harvested and analyzed in both groups, and then were histologically and immunohistochemically analyzed. Enzyme‐linked immunosorbent assay, Western blot analysis, and real‐time PCR were performed to evaluate the expression of interleukin‐6 (IL‐6), tumor necrosis factor α (TNF‐α), inducible nitric oxide synthase (iNOS), nuclear factor‐κB (NF‐κB) p65, IkB‐α, and activating transcription factor‐3 (ATF‐3). Results After 7 days of treatment, NPWT could obviously promote diabetic wound healing because of the mild inflammation and the dense cell‐deposited matrix. Meanwhile, NPWT significantly decreased the expression of TNF‐α, IL‐6, and iNOS (all P 
ISSN:1520-7552
1520-7560
DOI:10.1002/dmrr.2871