Temporal kinetics of CD8+CD28+ and CD8+CD28− T lymphocytes in the injured rat spinal cord

This study aims to explore the temporal changes of cytotoxic CD8+CD28+ and regulatory CD8+ CD28− T‐cell subsets in the lesion microenvironment after spinal cord injury (SCI) in rats, by combination of immunohistochemistry (IHC) and flow cytometry (FCM). In the sham‐opened spinal cord, few CD8+ T cel...

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Bibliographic Details
Published in:Journal of neuroscience research 2017-08, Vol.95 (8), p.1666-1676
Main Authors: Wu, Yan, Lin, Yu‐Hong, Shi, Ling‐Ling, Yao, Zong‐Feng, Xie, Xiu‐Mei, Jiang, Zheng‐Song, Tang, Jie, Hu, Jian‐Guo, Lü, He‐Zuo
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Annexin A5 - metabolism
Annexin V
Apoptosis
Apoptosis - physiology
CD28 antigen
CD28 Antigens - metabolism
CD8 antigen
CD8 Antigens - metabolism
CD8+T‐cell subsets
Cytometry
Cytotoxicity
Disease Models, Animal
Female
Flow Cytometry
Immunohistochemistry
Injuries
Kinetics
Lymphocytes
Lymphocytes T
microenvironment
Necrosis - etiology
Propidium iodide
Rats
Rats, Sprague-Dawley
Rope
Spinal cord
Spinal cord injuries
Spinal Cord Injuries - complications
Spinal Cord Injuries - immunology
Spinal Cord Injuries - pathology
spinal cord injury
Staining
Survival
T-Lymphocytes - physiology
Time Factors
Windows (intervals)
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Summary:This study aims to explore the temporal changes of cytotoxic CD8+CD28+ and regulatory CD8+ CD28− T‐cell subsets in the lesion microenvironment after spinal cord injury (SCI) in rats, by combination of immunohistochemistry (IHC) and flow cytometry (FCM). In the sham‐opened spinal cord, few CD8+ T cells were found. After SCI, the CD8+ T cells were detected at one day post‐injury (dpi), then markedly increased and were significantly higher at 3, 7, and 14 dpi compared with one dpi (p 
ISSN:0360-4012
1097-4547
DOI:10.1002/jnr.23993