Rapid selection of anti-hapten antibodies isolated from synthetic and semi-synthetic antibody phage display libraries expressed in Escherichia coli

Abstract Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin LR (1000 Da) conjugated to either bovine serum albumin or keyhole limpet haemocyanin. In com...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-05, Vol.210 (2), p.257-261
Main Authors: Strachan, G., McElhiney, J., Drever, M.R., McIntosh, F., Lawton, L.A., Porter, A.J.R.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - genetics
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - isolation & purification
Antibody fragment
Antibody libraries
Antibody Specificity
Antigens
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Binding, Competitive
Biological and medical sciences
Bovine serum albumin
Cloning, Molecular
E coli
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Hapten
Haptens
Haptens - chemistry
Haptens - immunology
Hemocyanins
Humans
Immunoassay
Immunoglobulins
Libraries
Microbiology
Microcystins
Monoclonal antibodies
Peptide Library
Peptides, Cyclic - chemistry
Peptides, Cyclic - immunology
Phage display
Phages
Route selection
Serum albumin
Serum Albumin, Bovine
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Abstract Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin LR (1000 Da) conjugated to either bovine serum albumin or keyhole limpet haemocyanin. In competition enzyme-linked immunosorbent assay, bacterially expressed antibodies selected via the Griffin library showed at least 300 times greater sensitivity than those isolated from the Tomlinson library, for free microcystin. Bacterially expressed phage antibody libraries provide a rapid and relatively easy route for the selection of monoclonal antibodies specific for even the most difficult of antigenic targets such as free haptens.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11190.x