The membrane-associated F0F1 ATPase is essential for the viability of Streptococcus pneumoniae

Abstract Genetic studies aimed at eliminating expression of the atp operon (F0F1 H+-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipien...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-06, Vol.212 (1), p.133-138
Main Authors: Ferrándiz, María José, de la Campa, Adela G
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
ATP
Bacterial diseases
Bacteriology
Biological and medical sciences
Chloramphenicol
Chloromycetin
Disruption
Essential gene
F0F1 H+‐ATPase
Fundamental and applied biological sciences. Psychology
H+-transporting ATPase
Human bacterial diseases
Infectious diseases
Medical sciences
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pneumococcus
Recombination
Staphylococcal infections, streptococcal infections, pneumococcal infections
Streptococcus infections
Streptococcus pneumoniae
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Summary:Abstract Genetic studies aimed at eliminating expression of the atp operon (F0F1 H+-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipient strain had a duplication of atpC, recombination occurring in such a way that transcription of the operon from its own promoter was allowed. These results imply that the atp operon is essential for the viability of the cells.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11256.x