Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides—relevance to AIDS vaccines in developing countries

Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine–phosphat...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2002-06, Vol.20 (21), p.2684-2692
Main Authors: Ayash-Rashkovsky, Mila, Weisman, Ziva, Diveley, Jocelyn, Moss, Ronald B., Bentwich, Zvi, Borkow, Gadi
Format: Article
Language:English
Subjects:
CpG ODN
HIV-1 immunogen
Human immunodeficiency virus 1
Schistosoma
Th1 and Th2 cytokine profile
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine–phosphate–guanosine (CpG) immunostimulatory sequences co-administered with inactivated, gp120-depleted HIV-1 viral particles (HIV-1 immunogen) lead to potent Th1 anti-HIV-1 immune responses overcoming the Th2 bias. In contrast, Schistosoma-infected mice immunized with HIV-1 immunogen in incomplete Freund’s adjuvant only, induced Th2 anti-HIV-1 immune responses. These findings strongly support the advisability of using CpG ODN as a Th1 inducing adjuvant when immunizing human populations with a strong pre-existent Th2 immune profile.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(02)00202-5