SIRP Alpha Protein Downregulates in Human Astrocytoma: Presumptive Involvement of Hsa-miR-520d-5p and Hsa-miR-520d-3p

Astrocytomas are the most common brain tumors with poor survival in malignant forms. Signal regulatory protein alpha (SIRP alpha) is a transmembrane protein expressed on immune cells and macrophages and is reported to modulate tumor cell phagocytosis. In the present study, we investigated the involv...

Full description

Saved in:
Bibliographic Details
Published in:Molecular neurobiology 2017-12, Vol.54 (10), p.8162-8169
Main Authors: Deshpande, Ravindra Pramod, Chandra Sekhar, Y.B.V.K, Panigrahi, Manas, Babu, Phanithi Prakash
Format: Article
Language:English
Subjects:
Adult
Antigens, Differentiation - genetics
Antigens, Differentiation - metabolism
Astrocytoma
Astrocytoma - genetics
Astrocytoma - metabolism
Astrocytoma - pathology
Binding sites
Biomarkers, Tumor
Biomedical and Life Sciences
Biomedicine
Brain cancer
Brain Neoplasms - genetics
Brain Neoplasms - metabolism
Brain Neoplasms - pathology
Brain tumors
Cell Biology
Computational neuroscience
Down-Regulation - physiology
Female
Gene expression
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Macrophages
Male
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
miRNA
Neurobiology
Neurology
Neurosciences
Phagocytosis
Protein expression
Proteins
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Tissues
Transcription
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Astrocytomas are the most common brain tumors with poor survival in malignant forms. Signal regulatory protein alpha (SIRP alpha) is a transmembrane protein expressed on immune cells and macrophages and is reported to modulate tumor cell phagocytosis. In the present study, we investigated the involvement of miR-520d-5p and miR-520d-3p in regulation of SIRP alpha expression. Here, we report mRNA and protein expression profile of SIRP alpha in 39 surgically resected human astrocytoma tissue samples and 14 control brain tissue samples. Transcript expression pattern was studied by real-time PCR while Western blotting and immunohistochemistry were used to evaluate protein expression. Expression profile of miR-520d-5p and miR-520d-3p was studied by real-time PCR. Computational prediction was employed to analyze the binding of miR-520d-5p and miR-520d-3p for SIRP alpha mRNA. It is evident from preliminary investigation that SIRP alpha transcripts are expressed in control brain tissues, increased in low-grade (grade II) tumor tissues, and decreased with further grade progression ( P  
ISSN:0893-7648
1559-1182
DOI:10.1007/s12035-016-0302-8