An osmosensing histidine kinase mediates dicarboximide fungicide resistance in Botryotinia fuckeliana ( Botrytis cinerea)

A two-component histidine protein kinase gene, homologous to os-1 from Neurospora crassa, was cloned and sequenced from a single ascospore isolate of Botryotinia fuckeliana. A series of nine spontaneous mutants resistant to dicarboximide fungicides was selected from this strain and characterized wit...

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Bibliographic Details
Published in:Fungal genetics and biology 2002-08, Vol.36 (3), p.187-198
Main Authors: Cui, Wei, Beever, Ross E, Parkes, Stephanie L, Weeds, Pauline L, Templeton, Matthew D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Botryotinia fuckeliana
Botrytis - drug effects
Botrytis - enzymology
Botrytis - genetics
Botrytis cinerea
Cyclins
Dicarboximide fungicides
DNA, Fungal
Drug Resistance, Microbial - genetics
Fungicides, Industrial - pharmacology
Genes, Fungal
Genome, Fungal
Histidine Kinase
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation
Neurospora crassa
Neurospora crassa - enzymology
Neurospora crassa - genetics
Osmotic Pressure
Osmotic sensitivity
Protein Kinases - genetics
Saccharomyces cerevisiae Proteins
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Summary:A two-component histidine protein kinase gene, homologous to os-1 from Neurospora crassa, was cloned and sequenced from a single ascospore isolate of Botryotinia fuckeliana. A series of nine spontaneous mutants resistant to dicarboximide fungicides was selected from this strain and characterized with respect to fungicide resistance and osmotic sensitivity. Genetic crosses of the mutants with an authentic Daf1 strain showed that the phenotypes mapped to this locus. Single point mutations (seven transitions, one transversion, and one short deletion) were detected in the alleles of the nine mutants sequenced. The mutational changes were shown to cosegregate with the dicarboximide resistance and osmotic sensitivity phenotypes in progeny obtained from crossing selected resistant strains with a sensitive strain. All mutations detected are predicted to result in amino acid changes in the coiled-coil region of the putative Daf1 histidine kinase, and it is proposed that dicarboximide fungicides target this domain.
ISSN:1087-1845
1096-0937
DOI:10.1016/S1087-1845(02)00009-9