Ionic liquids for the molecular enantiorecognition of free l-T sub(3), l-T sub(4) and d-T sub(4)

Ionic liquids were proposed for the molecular enantiorecognition of free l-T sub(3), l-T sub(4) and d-T sub(4). l-Phenylalanine tert-butyl ester lactate (l-PheC sub(4)-Lac), l-alanine tert-butyl ester l-lactate (l-AlaC sub(4)-Lac) and l-alanine tert-butyl ester nitrate (l-AlaC sub(4)-NO sub(3)) were...

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Bibliographic Details
Published in:RSC advances 2015-09, Vol.5 (92), p.75451-75457
Main Authors: Stefan-van Staden, Raluca-Ioana, Mitrofan, Grigorina, Comnea-Stancu, Ionela Raluca, van Staden, Jacobus Frederick, Kapnissi-Christodoulou, Constantina, Aboul-Enein, Hassan Y
Format: Article
Language:English
Subjects:
Assaying
Blood
Dissolution
Esters
Ionic liquids
Pretreatment
Sensors
Signatures
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Summary:Ionic liquids were proposed for the molecular enantiorecognition of free l-T sub(3), l-T sub(4) and d-T sub(4). l-Phenylalanine tert-butyl ester lactate (l-PheC sub(4)-Lac), l-alanine tert-butyl ester l-lactate (l-AlaC sub(4)-Lac) and l-alanine tert-butyl ester nitrate (l-AlaC sub(4)-NO sub(3)) were physically immobilized in a diamond paste. The best linear concentration range for the assay of free l-T sub(4) was obtained with the microsensor modified with l-AlaC sub(4)-NO sub(3) (1 10 super(-10)-1 10 super(-8) mol L super(-1)) while for the assay of free l-T sub(3) and free d-T sub(4), the microsensor based on l-PheC sub(4)-Lac provided the best linear ranges of 6 10 super(-12)-1 10 super(-11) mol L super(-1), and 1 10 super(-11)-1 10 super(-9) mol L super(-1), respectively. The signature of the analytes (the t sub(off) value) was different for each sensor, proving their enantiorecognition capacity. The main advantages of the constructed sensors are that the limits of determination achieved for free l-T sub(3) (6 10 super(-12) mol L super(-1)), free l-T sub(4) (1 10 super(-6) mol L super(-1)) and free d-T sub(4) (1 10 super(-11) mol L super(-1)) are low, the sample did not need any pretreatment before measurement, the assay is fast and reliable, and can be performed for both whole blood or pharmaceutical formulations without any sample pretreatment steps for whole blood samples, and by just dissolving the T sub(4) tablets in distilled water. The recoveries were higher than 99.00% for all thyroid hormones with RSD (%) values lower than 1.00%.
ISSN:2046-2069
DOI:10.1039/c5ra14635f