Genomic integration occurs in the packaging cell via unexported lentiviral precursors

Objective To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection. Results A co-transfection of a Human Embryonic Kidney (HEK) 293 packaging cell line with Gag–pol (GP) and a transfer vector, with...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2016-10, Vol.38 (10), p.1715-1721
Main Authors: Mosabbir, Abdullah Al, Truong, Kevin
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
cell lines
Cellular biology
DNA Packaging
Embryos
Envelopes
Formations
Fusion Proteins, gag-pol - genetics
Genes
Genetic Vectors
Genomics
HEK293 Cells
HIV-1 - genetics
Human immunodeficiency virus 1
Humans
Kidneys
Lentivirus
Life Sciences
Mice
Microbiology
Original Research Paper
Packaging
Precursors
Proteins
Transfection - methods
virion
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection. Results A co-transfection of a Human Embryonic Kidney (HEK) 293 packaging cell line with Gag–pol (GP) and a transfer vector, without the envelope vector, produces a stable cell line after 2 weeks of selection. Furthermore, a matrix protein deficient GP in the packaging vector enhances this integration. This supports that, in theory, unexported lentiviral cores produced within the packaging cell can infect itself without requiring the release of any lentiviral particles. Conclusion If the packaging cell is also the target cell, then gene integration leading to a stable cell line can be accomplished without viral particle infection.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-016-2164-6