A tandem cell for nanopore-based DNA sequencing with exonuclease

A tandem electrolytic cell with the structure [cis1, upstream nanopore (UNP), trans1 = cis2, downstream nanopore (DNP), trans2], an exonuclease enzyme attached to the downstream side of UNP, and a chemical adapter in or a profiled voltage over DNP can be used with bandwidths of a few kHz to sequence...

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Published in:RSC advances 2015-01, Vol.5 (1), p.167-171
Main Author: Sampath, G
Format: Article
Language:English
Subjects:
Adapters
Electric potential
Electrolytic cells
Enzymes
Nanostructure
Regression
Upstream
Voltage
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description A tandem electrolytic cell with the structure [cis1, upstream nanopore (UNP), trans1 = cis2, downstream nanopore (DNP), trans2], an exonuclease enzyme attached to the downstream side of UNP, and a chemical adapter in or a profiled voltage over DNP can be used with bandwidths of a few kHz to sequence bases in ssDNA in natural order with high accuracy and without loss in trans1/cis2 or regression into DNP from trans2.
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source Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)
subjects Adapters
Electric potential
Electrolytic cells
Enzymes
Nanostructure
Regression
Upstream
Voltage
title A tandem cell for nanopore-based DNA sequencing with exonuclease
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