Dengue diagnosis on laser printed microzones using smartphone-based detection and multivariate image analysis

This study describes the use of smartphone-based detection and multivariate image analysis (MIA) to perform dengue diagnosis on laser printed microzones. Toner-based microplates containing 96 microzones were fabricated on a transparency film surface through a single laser printing step. Enzyme-linke...

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Bibliographic Details
Published in:Analytical methods 2016-09, Vol.8 (35), p.656-6511
Main Authors: Oliveira, Karoliny A, Damasceno, Deangelis, de Oliveira, Cristina R, da Silveira, Lucimeire A, de Oliveira, Anselmo E, Coltro, Wendell K. T
Format: Article
Language:English
Subjects:
Dengue virus
Diagnosis
ELISA
Image analysis
Image detection
Immunoglobulins
Lasers
Patients
Viruses
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Summary:This study describes the use of smartphone-based detection and multivariate image analysis (MIA) to perform dengue diagnosis on laser printed microzones. Toner-based microplates containing 96 microzones were fabricated on a transparency film surface through a single laser printing step. Enzyme-linked-immunosorbent assay (ELISA) experiments were then carried out to detect both immunoglobulin M (IgM) and G (IgG) antibodies in serum samples collected from patients infected and not infected with dengue virus. Principal component analysis (PCA) of ELISA digital image data acquired with a smartphone revealed good discrimination between samples from infected and non-infected patients. Dengue diagnosis was performed on printed microzones based on colorimetric detection of IgG and IgM biomarkers in serum samples through enzyme-linked-immunosorbent assay experiments.
ISSN:1759-9660
1759-9679
DOI:10.1039/c6ay01262k