Guanidiniocarbonyl pyrrole (GCP) conjugated PAMAM-G2, a highly efficient vector for gene delivery: the importance of DNA condensation

A novel hybrid compound 1 efficiently shuttles genetic material into HeLa cells at concentrations as low as 0.6 μM, whereas the parent compound PAMAM-G2 is ineffective even at 200 μM. The high efficiency of 1 stems from its capabiliy to form highly condensed ligand-DNA polyplexes. Its binding affini...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (84), p.12446-12449
Main Authors: Samanta, Krishnananda, Jana, Poulami, Bäcker, Sandra, Knauer, Shirley, Schmuck, Carsten
Format: Article
Language:English
Subjects:
Affinity
Cationic
Dendrimers - chemistry
DNA - chemistry
Efficiency
Gene Transfer Techniques
Genes
Genetic Vectors
Genetics
Guanidines - chemistry
HeLa Cells
Humans
Parents
Polyethyleneimine
Polymers
Pyrroles - chemistry
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Summary:A novel hybrid compound 1 efficiently shuttles genetic material into HeLa cells at concentrations as low as 0.6 μM, whereas the parent compound PAMAM-G2 is ineffective even at 200 μM. The high efficiency of 1 stems from its capabiliy to form highly condensed ligand-DNA polyplexes. Its binding affinity is actually lower than for the parent dendrimer. Compound 1 is even 200 times more effective and less cytotoxic than PEI, the current gold standard in gene transfection with cationic polymers.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc06404c