A photochromic and thermochromic fluorescent protein

We report a photochromic and thermochromic fluorescent protein that exhibits a reversible and striking visible colour switch between yellow and red. The protein has been characterized in terms of its light, temperature and pH-dependence. Based on a mutational analysis we propose that the colour swit...

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Bibliographic Details
Published in:RSC advances 2014-01, Vol.4 (100), p.56762-56765
Main Authors: Shen, Y., Wiens, M. D., Campbell, R. E.
Format: Article
Language:English
Subjects:
Chromophores
Color
Colour
Fluorescence
Proteins
Protonation
Switches
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Summary:We report a photochromic and thermochromic fluorescent protein that exhibits a reversible and striking visible colour switch between yellow and red. The protein has been characterized in terms of its light, temperature and pH-dependence. Based on a mutational analysis we propose that the colour switch mechanism involves chromophore protonation coupled with E – Z isomerization.
ISSN:2046-2069
2046-2069
DOI:10.1039/C4RA10107C