Concave microwell plate facilitates chondrogenesis from mesenchymal stem cells

Objectives To compare in vitro chondrogenesis from bone marrow-derived mesenchymal stem cells using concave microwell plates with those obtained using culture tubes. Results Pellets cultured in concave microwell plates had a significantly higher level of GAG per DNA content and greater proteoglycan...

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Bibliographic Details
Published in:Biotechnology letters 2016-11, Vol.38 (11), p.1967-1974
Main Authors: Ko, Ji-Yun, Im, Gun-Il
Format: Article
Language:English
Subjects:
Aged
Applied Microbiology
Biochemistry
Biomass
Biomedical and Life Sciences
Biotechnology
Bone marrow
Bones
Cell Culture Techniques - instrumentation
Cell Differentiation
Cells, Cultured
Cellular biology
Chondrogenesis
Culture
DNA - analysis
Gene Expression Regulation
Humans
In vitro testing
Life Sciences
Markers
Mesenchymal Stromal Cells - cytology
Microbiology
Middle Aged
Original Research Paper
Pellets
Stem cells
Tubes
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Summary:Objectives To compare in vitro chondrogenesis from bone marrow-derived mesenchymal stem cells using concave microwell plates with those obtained using culture tubes. Results Pellets cultured in concave microwell plates had a significantly higher level of GAG per DNA content and greater proteoglycan content than those cultured in tubes at day 7 and 14. Three chondrogenic markers, SOX - 9 , COL2A1 and aggrecan , showed significantly higher expression in pellets cultured in concave microwell plates than those cultured in tubes at day 7 and 14. At day 21, there was not a significant difference in the expression of these markers. COL10A1 , the typical hypertrophy marker, was significantly lower in concave microwell plates during the whole culture period. Runx - 2 , a marker of hypertrophy and osteogenesis, was significantly lower at day 7 in pellets cultured in concave microwell plates than those cultured in tubes. Conclusion Concave microwell plates provide a convenient and effective tool for the study of in vitro chondrogenesis and may replace the use of propylene culture tube.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-016-2170-8