A new drug delivery system for Mitomycin C to improve intravesical instillation

The conventional administration of Mitomycin C (MMC) in intravesical instillations has its limitations. In this study, MMC was loaded onto an in situ forming depot consisting of chitosan (CS), β-glycerophosphate (GP) and Fe3O4 magnetic nanoparticles (Fe3O4-MNPs) to improve its effects. The features...

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Bibliographic Details
Published in:Materials & design 2016-11, Vol.110, p.849-857
Main Authors: Sun, Xiaoliang, Sun, Peng, Li, Bo, Liu, Yiqing, Wang, Muwen, Suo, Ning, Yang, Min, Zhang, Dong, Jin, Xunbo
Format: Article
Language:English
Subjects:
Apoptosis
Bladder
Chitosan
Drug delivery system
Drug delivery systems
Flow cytometry
In situ forming depot
Intravesical instillation
Metal matrix composites
Mitomycin C
Sol gel process
Sustained release
Transformations
Tumors
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Summary:The conventional administration of Mitomycin C (MMC) in intravesical instillations has its limitations. In this study, MMC was loaded onto an in situ forming depot consisting of chitosan (CS), β-glycerophosphate (GP) and Fe3O4 magnetic nanoparticles (Fe3O4-MNPs) to improve its effects. The features and effects of this new drug delivery mode were investigated. The new drug delivery system (Fe3O4-MMC-CS/GP) exhibited superior sol-gel transformation and magnetism. Sustained release of MMC was observed both in vitro and in vivo, and the retention, which lasted for 72h in the rat bladder, was further examined using frozen sections. The Cell-Counting Kit-8 (CCK-8) and flow cytometry assays revealed better anti-tumor activity of Fe3O4-MMC-CS/GP compared with the free MMC solution. Animal experiments showed that Fe3O4-MMC-CS/GP provided a favorable survival rate and inhibited the growth of bladder tumors. Moreover, this drug delivery system enhanced tumor cell apoptosis compared with the conventional route of MMC administration in rats. [Display omitted] •Mitomycin C was successfully loaded onto a new delivery system for intravesical instillation.•The new delivery system exhibited excellent sustained release and prolonged retention properties.•Both hematoxylin-eosin staining for retention and mimicry of urination were adequate and innovative experiment methods.•The anti-tumor and apoptotic effect of Mitomycin C combined with the new delivery system was significantly enhanced.
ISSN:0264-1275
1873-4197
DOI:10.1016/j.matdes.2016.08.058