Evolution of bacterial consortia in an integrated tannery wastewater treatment process

In the present study, the dynamics of microbial communities and their abundance associated with each stage of a tannery wastewater treatment process were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) combined with high throughput sequencing. Both PCR-DG...

Full description

Saved in:
Bibliographic Details
Published in:RSC advances 2016-01, Vol.6 (90), p.87380-87388
Main Authors: Liang, Hebin, Ye, Dongdong, Li, Pan, Su, Tingting, Wu, Jiegen, Luo, Lixin
Format: Article
Language:English
Subjects:
Abundance
Actinobacteria
Bacteria
Communities
Electrophoresis
Evolution
Firmicutes
Planctomycetes
Proteobacteria
Sequencing
Tanneries
Wastewater treatment
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, the dynamics of microbial communities and their abundance associated with each stage of a tannery wastewater treatment process were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) combined with high throughput sequencing. Both PCR-DGGE and high throughput sequencing results reflected the bacterial succession in the integrated treatment process and phyla of Proteobacteria, Bacteroidetes and Firmicutes dominated throughout the integrated treatment process. However, Actinobacteria, Planctomycetes and Chlofoflexi only predominant the anoxic/oxic (A/O) process. The bacterial richness during the A/O process was higher than that in the pre-treatment process. Moreover, quantitative polymerase chain reaction (qPCR) analysis indicated that the absolute abundance of 16S rRNA genes in the biological treatment stages were higher than in other stages. Finally, redundancy analysis suggested that [Thermi] should be involved in NH4+-N removal and ammonia concentration had positive effects on the bacterial diversity. Overall, this study provided insight into the evolution of the bacterial community structure and diversity in integrated wastewater treatment processes and identified the correlations between the physicochemical characteristics of wastewater and bacterial community structures.
ISSN:2046-2069
2046-2069
DOI:10.1039/c6ra19603a