HD Flow Cytometry: An Improved Way to Quantify Cellular Interactions with Nanoparticles

Histogram deconvolution flow cytometry enables improved quantification of nanomaterial–cell interactions. The algorithm identifies the positive cells in highly overlapped populations and calculates the fluorescence intensity of the positive population. This technique performs better than commerciall...

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Bibliographic Details
Published in:Advanced healthcare materials 2016-09, Vol.5 (18), p.2333-2338
Main Authors: Selby, Laura I., Kongkatigumjorn, Nachnicha, Such, Georgina K., Johnston, Angus P. R.
Format: Article
Language:English
Subjects:
Algorithms
Animals
cell interactions
deconvolution
Flow cytometry
Flow Cytometry - instrumentation
Flow Cytometry - methods
Fluorescence
Health care
Humans
Mathematical analysis
Nanoparticles
Nanostructure
Populations
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Summary:Histogram deconvolution flow cytometry enables improved quantification of nanomaterial–cell interactions. The algorithm identifies the positive cells in highly overlapped populations and calculates the fluorescence intensity of the positive population. This technique performs better than commercially available methods with the additional benefit of visualizing the output.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201600445