Two-way and three-way approaches to ultra high performance liquid chromatography-photodiode array dataset for the quantitative resolution of a two-component mixture containing ciprofloxacin and ornidazole

Two‐way and three‐way calibration models were applied to ultra high performance liquid chromatography with photodiode array data with coeluted peaks in the same wavelength and time regions for the simultaneous quantitation of ciprofloxacin and ornidazole in tablets. The chromatographic data cube (te...

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Bibliographic Details
Published in:Journal of separation science 2016-09, Vol.39 (18), p.3488-3497
Main Authors: Dinç, Erdal, Ertekin, Zehra Ceren, Büker, Eda
Format: Article
Language:English
Subjects:
Antibiotics
Arrays
Calibration
Chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - standards
Ciprofloxacin
Ciprofloxacin - analysis
Economic models
Factor analysis
High performance liquid chromatography
Infrared imaging systems
Least squares
Least squares method
Mathematical analysis
Ornidazole
Ornidazole - analysis
Regression analysis
Tablet analysis
Tablets
Tablets - analysis
Tensors
Three-way data analysis
Ultra high performance liquid chromatography
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Summary:Two‐way and three‐way calibration models were applied to ultra high performance liquid chromatography with photodiode array data with coeluted peaks in the same wavelength and time regions for the simultaneous quantitation of ciprofloxacin and ornidazole in tablets. The chromatographic data cube (tensor) was obtained by recording chromatographic spectra of the standard and sample solutions containing ciprofloxacin and ornidazole with sulfadiazine as an internal standard as a function of time and wavelength. Parallel factor analysis and trilinear partial least squares were used as three‐way calibrations for the decomposition of the tensor, whereas three‐way unfolded partial least squares was applied as a two‐way calibration to the unfolded dataset obtained from the data array of ultra high performance liquid chromatography with photodiode array detection. The validity and ability of two‐way and three‐way analysis methods were tested by analyzing validation samples: synthetic mixture, interday and intraday samples, and standard addition samples. Results obtained from two‐way and three‐way calibrations were compared to those provided by traditional ultra high performance liquid chromatography. The proposed methods, parallel factor analysis, trilinear partial least squares, unfolded partial least squares, and traditional ultra high performance liquid chromatography were successfully applied to the quantitative estimation of the solid dosage form containing ciprofloxacin and ornidazole.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201600382