Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats

• Published in: Journal of feline medicine and surgery 2017-10, Vol.19 (10), p.1091-1095
• Main Authors: Ackermann, Camila Louise, Trevisol, Eduardo, Crocomo, Leticia Ferrari, Rascado, Tatiana da Silva, Volpato, Rodrigo, Guaitolini, Carlos Renato de Freitas, Lopes, Carlize, Costa, Talita de Almeida, Lopes, Maria Denise
• Format: Article
• Language: English
• Subjects: Animals; Blastocyst - cytology; Blastocyst - physiology; Cats; Female; Fertilization in Vitro - methods; Fertilization in Vitro - veterinary; Oocyte Retrieval - methods; Oocyte Retrieval - veterinary; Oocytes - cytology; Oocytes - drug effects; Triptorelin Pamoate - analogs & derivatives; Triptorelin Pamoate - pharmacology
• ISSN: 1098-612X; 1532-2750; 1532-2750
• DOI: 10.1177/1098612X16680697

Objectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher’s exact test. All analyses were performed using GraphPad Prism v5.0, with P