Rapid test for detection of susceptibility to cefotaxime in Enterobacteriaceae

In this work an "in house" rapid test based on the change in pH that is due to hydrolysis for detecting Enterobacteriaceae susceptible to cefotaxime is evaluated. The strains of Enterobacteriaceae from 1947 urine cultures were assessed using MicroScan panels and the "in house" te...

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Bibliographic Details
Published in:Revista argentina de microbiología 2016-10, Vol.48 (4), p.320-324
Main Authors: Jiménez-Guerra, Gemma, Hoyos-Mallecot, Yannik, Rodríguez-Granger, Javier, Navarro-Marí, José María, Gutiérrez-Fernández, José
Format: Article
Language:Spanish
Subjects:
beta-Lactam Resistance
Cefotaxime - pharmacology
Colorimetry
Coloring Agents
Enterobacteriaceae - drug effects
Enterobacteriaceae - isolation & purification
Enterobacteriaceae Infections - microbiology
Escherichia coli Infections - microbiology
Escherichia coli Proteins - analysis
Hydrogen-Ion Concentration
Hydrolysis
Membrane Proteins - analysis
Microbial Sensitivity Tests - methods
Phenolsulfonphthalein
Predictive Value of Tests
Sensitivity and Specificity
Urinary Tract Infections - microbiology
Uropathogenic Escherichia coli - drug effects
Uropathogenic Escherichia coli - enzymology
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Summary:In this work an "in house" rapid test based on the change in pH that is due to hydrolysis for detecting Enterobacteriaceae susceptible to cefotaxime is evaluated. The strains of Enterobacteriaceae from 1947 urine cultures were assessed using MicroScan panels and the "in house" test. This rapid test includes red phenol solution and cefotaxime. Using MicroScan panels, 499 Enterobacteriaceae isolates were evaluated, which included 27 isolates of Escherichia coli producing extended-spectrum beta-lactamases (ESBL), 16 isolates of Klebsiella pneumoniae ESBL and 1 isolate of Klebsiella oxytoca ESBL. The "in house" test offers the following values: sensitivity 98% and specificity 97%, with negative predictive value 100% and positive predictive value 78%. The "in house" test based on the change of pH is useful in our area for detecting presumptively cefotaxime-resistant Enterobacteriaceae strains.
ISSN:0325-7541
DOI:10.1016/j.ram.2016.08.002