CURCUMIN IN MALE FERTILITY: EFFECTS ON SPERMATOZOA VITALITY AND OXIDATIVE BALANCE

The aim of this study was to assess the dose- and time-dependent effects of curcumin on bovine spermatozoa during short-term (0h, 2h, 6h) and long-term (12h, 24h) in vitro culture periods. Semen samples were collected from 20 adult breeding bulls, and diluted in physiological saline solution contain...

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Bibliographic Details
Published in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2015-02, Vol.4 (special issue 2 (Biotechnology)), p.120-124
Main Authors: Tvrdá, Eva, Lukáč, Norbert, Jambor, Tomáš, Lukáčová, Jana, Massányi, Peter
Format: Article
Language:English
Subjects:
Antioxidants
Fertility
Laboratories
Motility
Nitrogen dioxide
Physiology
Sperm
Spermatogenesis
Toxicity
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Summary:The aim of this study was to assess the dose- and time-dependent effects of curcumin on bovine spermatozoa during short-term (0h, 2h, 6h) and long-term (12h, 24h) in vitro culture periods. Semen samples were collected from 20 adult breeding bulls, and diluted in physiological saline solution containing 0.5% DMSO together with 0, 1, 5, 10, 50 and 100 μM/L of curcumin. Spermatozoa motion parameters were determined using the SpermVisionTM and CASA (Computer Assisted Semen Analyzer) system. Cell viability was measured using the metabolic activity MTT assay, and the nitroblue-tetrazolium (NBT) test was used to assess the intracellular superoxide formation. The CASA analysis revealed that concentrations of 50 μM/L and 10 μM/L of curcumin were able to significantly prevent the decrease of motility and progressive motility (P
ISSN:1338-5178
1338-5178
DOI:10.15414/jmbfs.2015.4.special2.120-124