Rice OsPBL1 (ORYZA SATIVA ARABIDOPSIS PBS1-LIKE 1) enhanced defense of Arabidopsis against Pseudomonas syringae DC3000

The receptor-like cytoplasmic kinases (RLCK family VII) are required for plant defense against various pathogens. Previously, OsPBL1 ( ORYZA SATIVA ARABIDOPSIS PBS1-LIKE 1 ) was isolated from rice as a potential RSV (rice stripe virus) resistant factor, but its physiological roles in plant defense a...

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Bibliographic Details
Published in:European journal of plant pathology 2016-12, Vol.146 (4), p.901-910
Main Authors: Khare, Ekta, Kim, Kangmin, Lee, Kui-Jae
Format: Article
Language:English
Subjects:
Agriculture
Arabidopsis
Biomedical and Life Sciences
Cytoplasm
Ecology
Hydrogen peroxide
Kinases
Life Sciences
Lycopersicon esculentum
Oryza sativa
Pathogens
Plant Pathology
Plant Sciences
Pseudomonas syringae
Rice
Tenuivirus
Tomatoes
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Summary:The receptor-like cytoplasmic kinases (RLCK family VII) are required for plant defense against various pathogens. Previously, OsPBL1 ( ORYZA SATIVA ARABIDOPSIS PBS1-LIKE 1 ) was isolated from rice as a potential RSV (rice stripe virus) resistant factor, but its physiological roles in plant defense are yet to be investigated. In this study, we demonstrated that OsPBL1 increased defense against P. syringae in transgenic Arabidopsis. To ascertain the role of OsPBL1 gene in plant defense, OsPBL1 tagged with HA (i.e. Hemagglutinin) was overexpressed in Arabidopsis and examined for the resistance against Pseudomonas syringae pv. tomato DC3000 (i.e. Pst DC3000). At 3 dpi of Pst DC3000, transgenic Arabidopsis lines exhibited the reduced chlorotic lesion and propagation of P. syringae , compared to wild type. Elevated pathogen resistance of transgenic lines was correlated with increased H 2 O 2 accumulation and callose deposition on the infected leaves. It was also revealed that expression levels of salicylic acid dependent genes such as PR1 , PR2 , and PR5 , were induced higher in transgenic lines than wild type. Taken together, our data suggested that OsPBL1 exerted the role in defense against pathogen attacks in plant via mainly facilitating salicylic acid dependent pathway.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-016-0968-9