Gelsolin role in microapocrine secretion

A role of gelsolin in opening the way along the microvilli for secretory vesicles during microapocrine secretion is proposed here. Data obtained with different techniques showed that many digestive enzymes are released by microapocrine secretion in insects. Proteins that might be involved in the mac...

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Bibliographic Details
Published in:Insect molecular biology 2016-12, Vol.25 (6), p.810-820
Main Authors: Silva, W., Ribeiro, A. F., Silva, M. C. P., Terra, W. R., Ferreira, C.
Format: Article
Language:English
Subjects:
Animals
Cloning, Molecular
DNA, Complementary - genetics
DNA, Complementary - metabolism
gelsolin
Gelsolin - genetics
Gelsolin - metabolism
Insect Proteins - genetics
Insect Proteins - metabolism
microapocrine secretion
RNA interference
secretory machinery
secretory vesicles
Sequence Analysis, DNA
Spodoptera - genetics
Spodoptera - metabolism
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Summary:A role of gelsolin in opening the way along the microvilli for secretory vesicles during microapocrine secretion is proposed here. Data obtained with different techniques showed that many digestive enzymes are released by microapocrine secretion in insects. Proteins that might be involved in the machinery of microapocrine secretion were selected from our transcriptomes and literature searches. The proteins were annexin, Complex actin‐related proteins 2 and 3 (ARP 2/3) cofilin, fimbrin, gelsolin 1, gelsolin 2, moesin, myosin 1, myosin 6, protein disulphide isomerase 1 (PDI 1), PDI 2 and profilin. The cDNAs coding for annexin, fimbrin, gelsolin 1, myosin 1, PDI 1 and PDI 2 were cloned and their sequences deposited in GenBank. Only gelsolin 1 and myosin 1 are expressed exclusively in the midgut (semiquantitative reverse transcriptase PCR). As myosin 1 may have a structural role in microvilli, gelsolin 1 is the best guess to be involved in the secretory machinery. A truncated recombinant gelsolin 1 was used to generate antibodies with which it was shown labelling inside and around midgut cell microvilli shown in an electron microscope, reinforcing a microvillar role for gelsolin 1. Suppression of gelsolin 1 synthesis by RNA interference prevents secretory vesicles from advancing inside the microvilli, in agreement with its putative role in severing the actin filaments to free the way for the vesicles.
ISSN:0962-1075
1365-2583
DOI:10.1111/imb.12265