Ghrelin-Induced Enhancement of Vasopressin and Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures

The effects of ghrelin on vasopressin and oxytocin secretion were studied in 13–14-day cell cultures of isolated rat neurohypophyseal tissue. The vasopressin and oxytocin contents of the supernatant were determined by radioimmunoassay after a 1- or 2-h incubation. Significantly increased levels of v...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2016-12, Vol.60 (4), p.525-530
Main Authors: Gálfi, M., Radács, M., Molnár, Zs, Budai, I., Tóth, G., Pósa, A., Kupai, K., Szalai, Z., Szabó, R., Molnár, H. A., Gardi, J., László, Ferenc A., Varga, Cs
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Blood-brain barrier
Cell Biology
Cell culture
Cell Line
Cells, Cultured
Education
Exocytosis
Gene expression
Ghrelin - pharmacology
Growth hormones
Hormones - pharmacology
Male
Neurochemistry
Neuroendocrine Cells - drug effects
Neuroendocrine Cells - metabolism
Neurology
Neurosciences
Oligopeptides - pharmacology
Oxytocin - metabolism
Peptides
Pituitary Gland - cytology
Pituitary Gland - metabolism
Proteomics
Rats
Rats, Wistar
Receptors, Ghrelin - antagonists & inhibitors
Receptors, Ghrelin - metabolism
Vasopressins - metabolism
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Summary:The effects of ghrelin on vasopressin and oxytocin secretion were studied in 13–14-day cell cultures of isolated rat neurohypophyseal tissue. The vasopressin and oxytocin contents of the supernatant were determined by radioimmunoassay after a 1- or 2-h incubation. Significantly increased levels of vasopressin and oxytocin production were detected in the cell culture media following ghrelin administration, depending on the ghrelin doses. The oxytocin level proved to be more elevated than that of vasopressin. The increase of vasopressin and oxytocin secretion could be totally blocked by previous administration of the ghrelin receptor antagonist ([D-Lys 3 ]-growth hormone-releasing peptide-6). Application of the ghrelin receptor antagonist after ghrelin administration proved ineffective. The results indicate that vasopressin and oxytocin release is influenced directly by the ghrelin system, and the effects of ghrelin on vasopressin and oxytocin secretion from the neurohypophyseal tissue in rats can occur at the level of the posterior pituitary. Our observations lend support to the view that neurohypophysis contains ghrelin receptors.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-016-0850-4