Simultaneous separation of apigenin, luteolin and rosmarinic acid from the aerial parts of the copper-tolerant plant Elsholtzia splendens

• Published in: Environmental science and pollution research international 2014, Vol.21 (13), p.8124-8132
• Main Authors: Peng, Hongyun, Xing, Yan, Gao, Lingling, Zhang, Liang, Zhang, Guolin
• Format: Article
• Language: English
• Subjects: Acetates; Acids; aerial parts; apigenin; Apigenin - analysis; Apigenin - chemistry; Apigenin - isolation & purification; Apigenin - toxicity; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biodegradation, Environmental; Biomass; Bioremediation; Cell Line, Tumor; China; Chromatography; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cinnamates - analysis; Cinnamates - chemistry; Cinnamates - isolation & purification; Cinnamates - toxicity; Copper; Cytotoxicity; Depsides - analysis; Depsides - chemistry; Depsides - isolation & purification; Depsides - toxicity; Earth and Environmental Science; Ecotoxicology; Elsholtzia; Elsholtzia splendens; Environment; Environmental Chemistry; Environmental Health; Ethanol; flowering; Flowers & plants; Flowers - chemistry; Fourier transform infrared spectroscopy; Fourier transforms; Humans; Lamiaceae - chemistry; Liquid chromatography; luteolin; Luteolin - analysis; Luteolin - chemistry; Luteolin - isolation & purification; Luteolin - toxicity; Magnetic Resonance Spectroscopy; Mass Spectrometry; Mining; Molecular Structure; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Plant Extracts - analysis; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - toxicity; Plant species; Polyamides; Prohibitins; Research Article; Rosmarinic Acid; Solvents; Spectroscopy, Fourier Transform Infrared; Studies; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-014-2747-5

Elsholtzia splendens is a copper-tolerant plant species which grows on copper deposits in China. The generation of a valuable E. splendens biomass on specific contaminated sites has become one of the promising phytotechnologies. The simultaneous separations of apigenin, luteolin, and rosmarinic acid yielded in the ethyl acetate extracts of the flowering aerial parts was achieved by the use of a macroporous resin, polyamide, and silicagel columns during chromatography. Chemical identification confirmed the structures based on the spectra of FTIR, NMR, and HPLC/ESI-MS. The isolated compounds of purity above 98.3 % were evaluated for their in vitro cytotoxic activities against human cancer cell lines including A549 (non-small lung), A431 (skin), and Bcap37 (breast). Among these compounds, luteolin and apigenin presented the best cytotoxic activities against A549, A431, and Bcap37 cells and, therefore, both could be the valuable products for the post-harvest processing of E. splendens biomass.