LPS-Induced Macrophage Activation and Plasma Membrane Fluidity Changes are Inhibited Under Oxidative Stress

• Published in: The Journal of membrane biology 2016-12, Vol.249 (6), p.789-800
• Main Authors: de la Haba, Carlos, Morros, Antoni, Martínez, Paz, Palacio, José R.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Cell Line; Cell Membrane - metabolism; Cell Survival - drug effects; Cells; Fluid dynamics; Human Physiology; Humans; Hydrogen Peroxide - pharmacology; Life Sciences; Lipopolysaccharides - immunology; Macrophage Activation - immunology; Macrophages - immunology; Macrophages - metabolism; Membrane Fluidity - immunology; Membrane Microdomains - metabolism; Membranes; Oxidation-Reduction; Oxidative Stress; Plasma
• ISSN: 0022-2631; 1432-1424
• DOI: 10.1007/s00232-016-9927-9

Macrophage activation is essential for a correct and efficient response of innate immunity. During oxidative stress membrane receptors and/or membrane lipid dynamics can be altered, leading to dysfunctional cell responses. Our aim is to analyze membrane fluidity modifications and cell function under oxidative stress in LPS-activated macrophages. Membrane fluidity of individual living THP-1 macrophages was evaluated by the technique two-photon microscopy. LPS-activated macrophage function was determined by TNFα secretion. It was shown that LPS activation causes fluidification of macrophage plasma membrane and production of TNFα. However, oxidative stress induces rigidification of macrophage plasma membrane and inhibition of cell activation, which is evidenced by a decrease of TNFα secretion. Thus, under oxidative conditions macrophage proinflammatory response might develop in an inefficient manner.