Protective Effects of Intralipid and Caffeic Acid Phenethyl Ester (CAPE) on Hepatotoxicity and Pancreatic Injury Caused by Dichlorvos in Rats

• Published in: Biochemical genetics 2016-12, Vol.54 (6), p.803-815
• Main Authors: Alp, Harun, Pinar, Neslihan, Dokuyucu, Recep, Sahan, Mustafa, Oruc, Cem, Kaplan, Ibrahim, Senol, Serkan, Ceyran, Ayse Bahar
• Format: Article
• Language: English
• Subjects: Animal tissues; Animals; Antioxidants; Biochemistry; Biomedical and Life Sciences; Biomedicine; Caffeic Acids - administration & dosage; Caffeic Acids - pharmacology; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - prevention & control; Dichlorvos - toxicity; Edema; Emulsions - administration & dosage; Emulsions - pharmacology; Gene Expression Regulation - drug effects; Hepatotoxicity; Human Genetics; Intoxication; Male; Medical Microbiology; Original Article; Oxidative stress; Oxidative Stress - drug effects; Oxidizing agents; Pancreatic Diseases - chemically induced; Pancreatic Diseases - metabolism; Pancreatic Diseases - prevention & control; Pesticides; Phenylethyl Alcohol - administration & dosage; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacology; Phospholipids - administration & dosage; Phospholipids - pharmacology; Rats; Rats, Wistar; Soybean Oil - administration & dosage; Soybean Oil - pharmacology; Treatment Outcome; Zoology
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-016-9757-6

The present study was aimed to the investigate the protective effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity and pancreatic injury caused by acute dichlorvos (D) intoxication in rats. Forty-eight Wistar rats were randomly divided into seven groups each containing seven rats except control groups. The groups included control, D, CAPE, IL, D + CAPE, D + IL, and D + CAPE + IL. Total antioxidant status and total oxidative stress levels were measured by automated colorimetric assay. Tissues were evaluated using hematoxylin and eosin (H&E) staining. Tissues were analyzed with hematoxylin and eosin by using standard protocols. Also, Bcl-2, Bax and caspase-3 were evaluated by immunohistochemical method in liver tissue. Total oxidant status in control, CAPE, and IL groups were significantly lower, and total antioxidant status in the D + CAPE, D + IL, and D + IL + CAPE groups were significantly higher compared to the D group. CAPE and IL treatment decreased the apoptotic and mitotic cell count in liver tissue. Parenchymal necrosis caused by dichlorvos is observed in pancreas tissues of rats. Mild congestion and edema formation occurred in pancreas tissues following D + CAPE and D + IL therapies. These results indicate that CAPE and IL have the potential to decrease oxidative stress and hepatic and pancreatic injuries caused by acute dichlorvos intoxication. These drugs can be considered as a new method for supportive and protective therapy against pesticide intoxication.