Genomic and phenotypic analyses of Serratia fonticola strain GS2: a rhizobacterium isolated from sesame rhizosphere that promotes plant growth and produces N-acyl homoserine lactone

• Published in: Journal of biotechnology 2017-01, Vol.241, p.158-162
• Main Authors: Jung, Byung Kwon, Khan, Abdur Rahim, Hong, Sung-Jun, Park, Gun-Seok, Park, Yeong-Jun, Park, Chang Eon, Jeon, Hwang-Ju, Lee, Sung-Eun, Shin, Jae-Ho
• Format: Article
• Language: English
• Subjects: 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - analysis; 4-Butyrolactone - chemistry; 4-Butyrolactone - metabolism; Chromatography, High Pressure Liquid; Genome, Bacterial - genetics; Phenotype; Plant growth-promoting rhizobacteria; Plasmids; Quorum Sensing; Rhizosphere; Serratia - genetics; Serratia - metabolism; Serratia fonticola
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2016.12.002

•Complete genome sequence of Serratia fonticola strain GS2 consists of a single circular chromosome and two circular plasmids.•Plant growth-promoting traits and beneficial quorum-sensing related genes were identified in the genome of Serratia fonticola strain GS2.•N-acyl homoserine lactone-production and plant growth-promoting traits of Serratia fonticola strain GS2 were confirmed through biochemical analysis. The genus Serratia incorporates many agriculturally important species. Serratia fonticola strain GS2, isolated from a sesame rhizosphere, can produce N-acyl homoserine lactone quorum sensing signal molecules and indole-3-acetic acid (IAA). Here we report the complete genome sequence and phenotypic characteristics based on genomic information of this bacterium. The complete genome sequence of S. fonticola strain GS2 consists of a chromosome of 6.1 Mbp and two plasmids of 132 kbp and 94 kbp. The genome clusters for IAA and N-acyl homoserine lactone biosynthesis were identified in the genome. Subsequently, the Salkowski test and gas chromatography-mass spectrometry (GC–MS) analysis also revealed that the strain GS2 produces indolic compounds (27.1μgmL−1) and IAA (6.7μgmL−1) in its culture broth. HPLC analysis confirmed that the strain GS2 produced the quorum sensing signal molecules N-hexanoyl-L-homoserine lactone and N-octanoyl-L-homoserine lactone. This new information on the genome sequence and phenotype features will inform future ecological studies related to plant-microbe interactions.