A polymerase chain reaction (PCR) application for free-living nematodes (Rhabditida) discrimination

Synthetic primers were designed and constructed based on Caenorhabditis elegans genomic sequences, targeting HSP70 related sequences. Genomic DNA derived from several free-living bacterial feeding nematode species was subjected to polymerase chain reaction (PCR), attempting discrimination among them...

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Bibliographic Details
Published in:Molecular ecology notes 2002-09, Vol.2 (3), p.248-249
Main Authors: Nikolaidis, N., Scouras, Z. G.
Format: Article
Language:English
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Summary:Synthetic primers were designed and constructed based on Caenorhabditis elegans genomic sequences, targeting HSP70 related sequences. Genomic DNA derived from several free-living bacterial feeding nematode species was subjected to polymerase chain reaction (PCR), attempting discrimination among them. The amplified DNA fragments exhibited a distinct and reproducible pattern that characterizes different nematode species and populations.
ISSN:1471-8278
1471-8286
DOI:10.1046/j.1471-8286.2002.00215.x