Induction of stress shock proteins DnaK and GroEL by phenoxyherbicide 2,4-D in Burkholderia sp. YK-2 isolated from rice field

The purpose of this work was to investigate the induction of stress shock proteins in Burkholderia sp. YK-2 in response to the phenoxyherbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The stress shock proteins, which contribute to the resistance of the cytotoxic effect of 2,4-D, were induced at diff...

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Bibliographic Details
Published in:Current microbiology 2000-07, Vol.41 (1), p.33-38
Main Authors: CHO, Y.-S, PARK, S.-H, KIM, C.-K, OH, K.-H
Format: Article
Language:English
Subjects:
2,4-Dichlorophenoxyacetic Acid - metabolism
2,4-Dichlorophenoxyacetic Acid - pharmacology
Applied sciences
Bacterial Proteins - analysis
Bacterial Proteins - metabolism
Bacteriology
Biodegradation, Environmental
Biological and medical sciences
Biological and physicochemical properties of pollutants. Interaction in the soil
Blotting, Western
Burkholderia
Burkholderia - genetics
Burkholderia - metabolism
Chaperonin 60 - biosynthesis
Chaperonin 60 - genetics
Chaperonin 60 - metabolism
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Escherichia coli Proteins
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Grasses
Grasslands
Herbicides - metabolism
Herbicides - pharmacology
HSP70 Heat-Shock Proteins - biosynthesis
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Metabolism. Enzymes
Microbiology
Oryza - microbiology
Oryza sativa
Pollution
Proteins
Public safety
Soil and sediments pollution
Soil Microbiology
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Summary:The purpose of this work was to investigate the induction of stress shock proteins in Burkholderia sp. YK-2 in response to the phenoxyherbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The stress shock proteins, which contribute to the resistance of the cytotoxic effect of 2,4-D, were induced at different 2,4-D concentrations in exponentially growing cultures of Burkholderia sp. YK-2. This response involved the induction of a 43-kDa DnaK and 41-kDa GroEL proteins, characterized by SDS-PAGE and Western blot by use of the anti-DnaK and anti-GroEL monoclonal antibodies. The total stress shock proteins were analyzed by 2-D PAGE. Survival of Burkholderia sp. YK-2 with time in the presence of different concentrations of 2,4-D was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002840010087