Isolation and characterization of microsatellites in Pacific white shrimp Penaeus (Litopenaeus) vannamei

Five polymorphic microsatellite loci were characterized for Penaeus (Litopenaeus) vannamei. Loci were isolated using a partial Sau3A1 genomic library by the sequencing of randomly selected clones and by a biotinylated (CT) sub(10) and (GT) sub(10) probes screening procedure. The last strategy result...

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Bibliographic Details
Published in:Molecular ecology notes 2002-09, Vol.2 (3), p.239-241
Main Authors: Cruz, P., Mejia-Ruiz, C. H., Perez-Enriquez, R., Ibarra, A. M.
Format: Article
Language:English
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Summary:Five polymorphic microsatellite loci were characterized for Penaeus (Litopenaeus) vannamei. Loci were isolated using a partial Sau3A1 genomic library by the sequencing of randomly selected clones and by a biotinylated (CT) sub(10) and (GT) sub(10) probes screening procedure. The last strategy resulted in the most useful data. About 40% of the clones showed a previously reported satellite/microsatellite (PVS1), reducing the chance of finding new microsatellite regions. Whereas two of the microsatellite loci with more than 10 alleles will be useful for mating analysis in a breeding program, the others might prove useful for population genetic studies.
ISSN:1471-8278
1471-8286
DOI:10.1046/j.1471-8286.2002.00211.x