Detection of differentially expressed genes in broiler pectoralis major muscle affected by White Striping - Wooden Breast myopathies

• Published in: Poultry science 2016-12, Vol.95 (12), p.2771-2785
• Main Authors: Zambonelli, Paolo, Zappaterra, Martina, Soglia, Francesca, Petracci, Massimiliano, Sirri, Federico, Cavani, Claudio, Davoli, Roberta
• Format: Article
• Language: English
• Subjects: Animals; Chickens - metabolism; Electrophoresis, Polyacrylamide Gel - veterinary; Gene Expression - physiology; Male; Muscle Development - genetics; Muscle Proteins - metabolism; Muscular Diseases - metabolism; Muscular Diseases - veterinary; Oligonucleotide Array Sequence Analysis - veterinary; Pectoralis Muscles - metabolism; Poultry Diseases - metabolism; Real-Time Polymerase Chain Reaction - veterinary
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps/pew268

White Striping and Wooden Breast (WS/WB) are abnormalities increasingly occurring in the fillets of high breast yield and growth rate chicken hybrids. These defects lead to consistent economic losses for poultry meat industry, as affected broiler fillets present an impaired visual appearance that negatively affects consumers' acceptability. Previous studies have highlighted in affected fillets a severely damaged muscle, showing profound inflammation, fibrosis, and lipidosis. The present study investigated the differentially expressed genes and pathways linked to the compositional changes observed in WS/WB breast muscles, in order to outline a more complete framework of the gene networks related to the occurrence of this complex pathological picture. The biochemical composition was performed on 20 pectoralis major samples obtained from high breast yield and growth rate broilers (10 affected vs. 10 normal) and 12 out of the 20 samples were used for the microarray gene expression profiling (6 affected vs. 6 normal). The obtained results indicate strong changes in muscle mineral composition, coupled to an increased deposition of fat. In addition, 204 differentially expressed genes (DEG) were found: 102 up-regulated and 102 down-regulated in affected breasts. The gene expression pathways found more altered in WS/WB muscles are those related to muscle development, polysaccharide metabolic processes, proteoglycans synthesis, inflammation, and calcium signaling pathway. On the whole, the findings suggest that a multifactorial and complex etiology is associated with the occurrence of WS/WB muscle abnormalities, contributing to further defining the transcription patterns associated with these myopathies.