Presence and distribution of yeasts in the reproductive tract in healthy female horses

• Published in: Equine veterinary journal 2017-09, Vol.49 (5), p.614-617
• Main Authors: Azarvandi, A., Khosravi, A. R., Shokri, H., Talebkhan Garoussi, M., Gharahgouzlou, F., Vahedi, G., Sharifzadeh, A.
• Format: Article
• Language: English
• Subjects: Agar; Animals; Candida; Candida - classification; Candida - isolation & purification; Colonies; Colony Count, Microbial - veterinary; Culture Media; Female; Females; Fungi; genital system; Genitalia, Female - microbiology; horse; Horses; Horses - microbiology; Longitudinal Studies; Mucosa; multiparous animal; Reproductive system; Skin; Vagina; yeast; Yeasts; Yeasts - classification; Yeasts - isolation & purification
• ISSN: 0425-1644; 2042-3306
• DOI: 10.1111/evj.12657

Summary Background Yeasts are commensal organisms found in the reproductive and gastrointestinal tracts, and on the skin and other mucosa in mammals. Objectives The purpose of this study was to isolate and identify yeast flora in the caudal reproductive tract in healthy female horses. Study design Longitudinal study. Methods A total of 453 samples were collected using double‐guarded swabs from the vestibule, clitoral fossa and vagina in 151 horses. All samples were cultured on Sabouraud 4% dextrose agar and incubated at 35°C for 7–10 days. Isolates were identified according to their morphological characteristics and biochemical profiles. Results Yeast colonies were isolated from 60 (39.7%) of the 151 horses. The isolated yeasts belonged to nine genera, and included Candida spp. (53.2%), Cryptococcus spp. (12.2%), Saccharomyces spp. (10.5%), Geotrichum spp. (8.0%), Rhodotorula spp. (7.1%), Malassezia spp. (3.7%), Trichosporon spp. (2.6%), Kluyveromyces spp. (2.6%) and Sporothrix spp. (0.2%). Candida krusei (43.1%) was the most frequent Candida species isolated. There was a significant difference in prevalence between C. krusei and other Candida species (P