Cloning of two new cry genes from Bacillus thuringiensis subsp. wuhanensis strain

With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8-78.7% identical to that of Cry1B group. The nom...

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Bibliographic Details
Published in:Current microbiology 2000-04, Vol.40 (4), p.227-232
Main Authors: KUO, W.-S, LIN, J.-H, TZENG, C.-C, KAO, S.-S, CHAK, K.-F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Bacillus thuringiensis
Bacillus thuringiensis - chemistry
Bacillus thuringiensis - genetics
Bacterial Proteins - genetics
Bacterial Proteins - toxicity
Bacterial Toxins
Bacteriology
Base Sequence
Biological and medical sciences
Butterflies - drug effects
Cloning
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetics
Larva - drug effects
Larvae
Lethal Dose 50
Microbiology
Molecular Sequence Data
Moths - drug effects
Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
Toxins, Biological - genetics
Toxins, Biological - toxicity
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Summary:With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8-78.7% identical to that of Cry1B group. The nomenclature assignment of these two clones is, therefore, named Cry1Gb1 and Cry1Bd1, respectively. The Cry1Bd1 is toxic to Plutella xylostella larvae, and the Cry1Gb1 is toxic to Pieris rapae larvae.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849910046