Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/ umu-test) and improvement of mutagen-sensitivity

Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2001, Vol.92 (3), p.301-304
Main Authors: Arai, Ryoichi, Makita, Yukimasa, Oda, Yoshimitsu, Nagamune, Teruyuki
Format: Article
Language:English
Subjects:
Biological and medical sciences
chimeric promoter
FLUORESCENCE
General aspects. Methods
GENES
GENOTOXICITY
green fluorescent protein (GFP)
Medical sciences
MUTAGENS
PROTEINS
recA mutant
tandem promoter
Toxicology
umu-test
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Summary:Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu promoter, thereby improving the sensitivity of the umu-test.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(01)80267-X